Isolation and comparison of α- and β-D-glucans from shiitake mushrooms (Lentinula edodes) with different biological activities

A polysaccharide-enriched extract obtained from Lentinula edodes was submitted to several purification steps to separate three different D-glucans with β-(1→6), β-(1→3),(1→6) and α-(1→3) linkages, being characterized through GC–MS, FT-IR, NMR, SEC and colorimetric/fluorimetric determinations. Moreover, in vitro hypocholesterolemic, antitumoral, anti-inflammatory and antioxidant activities were also tested. Isolated glucans exerted HMGCR inhibitory activity, but only β-(1→6) and β-(1→3),(1→6) fractions showed DPPH scavenging capacity. Glucans were also able to lower IL-1β and IL-6 secretion by LPS-activated THP-1/M cells and showed cytotoxic effect on a breast cancer cell line that was not observed on normal breast cells. These in vitro results pointed important directions for further in vivo studies, showing different effects of each chemical structure of the isolated glucans from shiitake mushrooms.

Bibliographic Details

Main Authors:	Morales, Diego, Rutckeviski, Renata, Villalva, Marisol, Abreu, Hellen, Soler-Rivas, Cristina, Santoyo, Susana, Iacomini, Marcello, Ribeiro Smiderle, Fhernanda
Other Authors:	Ministerio de Economía y Competitividad (España)
Format:	artículo biblioteca
Language:	English
Published:	Elsevier 2020
Subjects:	Shiitake mushroom, Cytotoxic, Hypocholesterolemic, Anti-inflammatory,
Online Access:	http://hdl.handle.net/10261/220149 http://dx.doi.org/10.13039/501100002322 http://dx.doi.org/10.13039/501100004612 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100003593 http://dx.doi.org/10.13039/100012818
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