Fast capillary electrophoresis method for determination of docosahexaenoic and eicosapentaenoic acids in marine oils omega-3 supplements
Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are essential omega-3 fatty acids (FA) in human nutrition. Dietary supplements containing these FA are available in the market and there is a need to assess their quality to prevent frauds. The purpose of this work was to optimize and validate a fast capillary electrophoresis (CE) method to determine EPA and DHA content in marine oils omega-3 supplements. Sample preparation comprised only a saponification step and analysis time was 8 min. Validation was performed according to limits of detection and quantification, linearity, accuracy and repeatability. Ten real samples of marine oil supplements were analyzed by CE and compared to conventional gas chromatography method. No significant differences between both methods were found within 95% confidence interval. Overall, CE method was successful to FA quantification and it could be used for fast FA monitoring during omega-3 supplement manufacture, final product quality assurance and labelling.
Main Authors: | , , , , |
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Other Authors: | |
Format: | artículo biblioteca |
Language: | English |
Published: |
Elsevier
2020
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Subjects: | Capillary electrophoresis, Fish oil, Ultraviolet detector, Krill oil, |
Online Access: | http://hdl.handle.net/10261/220099 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100006366 http://dx.doi.org/10.13039/501100003593 http://dx.doi.org/10.13039/501100002322 |
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