Fast capillary electrophoresis method for determination of docosahexaenoic and eicosapentaenoic acids in marine oils omega-3 supplements

Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are essential omega-3 fatty acids (FA) in human nutrition. Dietary supplements containing these FA are available in the market and there is a need to assess their quality to prevent frauds. The purpose of this work was to optimize and validate a fast capillary electrophoresis (CE) method to determine EPA and DHA content in marine oils omega-3 supplements. Sample preparation comprised only a saponification step and analysis time was 8 min. Validation was performed according to limits of detection and quantification, linearity, accuracy and repeatability. Ten real samples of marine oil supplements were analyzed by CE and compared to conventional gas chromatography method. No significant differences between both methods were found within 95% confidence interval. Overall, CE method was successful to FA quantification and it could be used for fast FA monitoring during omega-3 supplement manufacture, final product quality assurance and labelling.

Bibliographic Details

Main Authors:	Amorim, Tatiane Lima, Mattos Duarte, Lucas, Fuente, Miguel Ángel de la, Leal de Oliveira, Marcone Augusto, Gómez-Cortés, Pilar
Other Authors:	Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil)
Format:	artículo biblioteca
Language:	English
Published:	Elsevier 2020
Subjects:	Capillary electrophoresis, Fish oil, Ultraviolet detector, Krill oil,
Online Access:	http://hdl.handle.net/10261/220099 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100006366 http://dx.doi.org/10.13039/501100003593 http://dx.doi.org/10.13039/501100002322
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