Peptides identified in egg white gastrointestinal digest as inducers of anorexigenic hormones in STC-1 cells

Peptides identified in egg white gastrointestinal digest as inducers of anorexigenic hormones in STC-1 cells

Resumen del trabajo presentado al 1st Ibero-American Congress on Bioactive Peptides (CIAPep), celebrado en Campinas-SP (Brasil) del 3 al 5 de diciembre de 2019.

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Main Authors: Santos-Hernández, Marta, Amigo, Lourdes, Miralles, Beatriz, Recio, Isidra
Other Authors: Ministerio de Economía y Competitividad (España)
Format: póster de congreso biblioteca
Language:English
Published: 2019
Online Access:http://hdl.handle.net/10261/205134
http://dx.doi.org/10.13039/501100003329
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spelling dig-cial-es-10261-2051342020-03-26T02:24:49Z Peptides identified in egg white gastrointestinal digest as inducers of anorexigenic hormones in STC-1 cells Santos-Hernández, Marta Amigo, Lourdes Miralles, Beatriz Recio, Isidra Ministerio de Economía y Competitividad (España) Resumen del trabajo presentado al 1st Ibero-American Congress on Bioactive Peptides (CIAPep), celebrado en Campinas-SP (Brasil) del 3 al 5 de diciembre de 2019. Dietary proteins are potent inducers of satiety and suppress short-term food intake. Protein digestion products can actas pre-absorptive signaling molecules, able to actívate enteroendocrine cells which release a variety of hormones, such as CCK or GLP-1. These hormones modulate the progress of the digestion and satiety. The aim of this work was to investigate the effects of egg white gastrointestinal digests and specific peptides released during digestion on CCK and GLP-1 secretion in enteroendocrine cells. Digests were characterized by mass spectrometry and amino acid analysis and assayed in STC-1 cells. This enteroendocrine cellular model had been previously validated with human jejunal digests in our research group. At the end of the egg white gastrointestinal digestion, 28% of the total nitrogen content corresponded to free amino acids, while the rest was in the form of peptides and intact protein. In fact, traces of ovalbumin and ovalbumin-Y were still detected by SDS-PAGE at the end of the digestion process. In arder to identify the molecules involved in hormonal secretion, intestinal digests were fractionated by size exclusion chromatography (SEC) into three different fractions: > 3 kDa, 3-0.5 kDa and <0.5 kDa, In addition, purified proteins and mixture of free a mino acids were also assayed in this cell line. The highest GLP-1 response was obtained with intestinal digests and SEC-intestinal fractions containing medium-size peptides, between 0.5 and 3 kDa, whereas a mixture of free ami no acids did not induce the release of this hormone. The mixture of free amino acids induced only a weak CCK secretion/which was maximized with peptides1 although peptide size did not clearly influenced CCK response. Interestingly, peptide sequences triggering CCK secretion showed different structural characteristics than those inducing GLP-1 release. This work opens new insights into the field of nutrient sensing at the gastrointestinal tract. This work has received financial support from Ministry of Economy and Competitiveness (MINECO, Spain) through the project AGL2015-66886-R. Peer reviewed 2020-03-25T12:16:01Z 2020-03-25T12:16:01Z 2019 póster de congreso http://purl.org/coar/resource_type/c_6670 1st Ibero-American Congress on Bioactive Peptides (2019) http://hdl.handle.net/10261/205134 http://dx.doi.org/10.13039/501100003329 en #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2015-66886-R Sí none
institution CIAL ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-cial-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del CIAL España
language English
description Resumen del trabajo presentado al 1st Ibero-American Congress on Bioactive Peptides (CIAPep), celebrado en Campinas-SP (Brasil) del 3 al 5 de diciembre de 2019.
author2 Ministerio de Economía y Competitividad (España)
author_facet Ministerio de Economía y Competitividad (España)
Santos-Hernández, Marta
Amigo, Lourdes
Miralles, Beatriz
Recio, Isidra
format póster de congreso
author Santos-Hernández, Marta
Amigo, Lourdes
Miralles, Beatriz
Recio, Isidra
spellingShingle Santos-Hernández, Marta
Amigo, Lourdes
Miralles, Beatriz
Recio, Isidra
Peptides identified in egg white gastrointestinal digest as inducers of anorexigenic hormones in STC-1 cells
author_sort Santos-Hernández, Marta
title Peptides identified in egg white gastrointestinal digest as inducers of anorexigenic hormones in STC-1 cells
title_short Peptides identified in egg white gastrointestinal digest as inducers of anorexigenic hormones in STC-1 cells
title_full Peptides identified in egg white gastrointestinal digest as inducers of anorexigenic hormones in STC-1 cells
title_fullStr Peptides identified in egg white gastrointestinal digest as inducers of anorexigenic hormones in STC-1 cells
title_full_unstemmed Peptides identified in egg white gastrointestinal digest as inducers of anorexigenic hormones in STC-1 cells
title_sort peptides identified in egg white gastrointestinal digest as inducers of anorexigenic hormones in stc-1 cells
publishDate 2019
url http://hdl.handle.net/10261/205134
http://dx.doi.org/10.13039/501100003329
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AT mirallesbeatriz peptidesidentifiedineggwhitegastrointestinaldigestasinducersofanorexigenichormonesinstc1cells
AT recioisidra peptidesidentifiedineggwhitegastrointestinaldigestasinducersofanorexigenichormonesinstc1cells
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