Vitamin D-enriched extracts obtained from shiitake mushrooms (Lentinula edodes) by supercritical fluid extraction and UV-irradiation
The combination of supercritical fluid extraction followed by UV-irradiation resulted in an interesting strategy to obtain ergosterol- and Vitamin D2-enriched extracts from Lentinula edodes to design novel functional food. Extractions carried out following a specific experimental design pointed out that extraction yields and ergosterol concentrations were more influenced by pressure than by the extraction temperature although, high temperatures (55–75 °C) might induce transformation of ergosta-7,22-dienol and fungisterol into ergosterol. After extraction, the ergosterol-enriched extracts should be dissolved in methanol or ethanol and irradiated (25 °C, 4 cm) to partially transform ergosterol into vitamin D2. Irradiation at 365 nm was less effective than at 254 nm. The fastest transformation was obtained using a UV lamp covering the complete UV spectrum for a maximum of 1 h. However, this lamp also induced vitamin D4 formation although in lower amounts than vitamin D2 or lumisterol2 while with irradiation at 254 nm most of ergosterol was transformed into vitamin D2.
| Main Authors: | , , , , , |
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| Other Authors: | |
| Format: | artículo biblioteca |
| Language: | English |
| Published: |
Elsevier
2017
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| Subjects: | Supercritical fluid extraction, Cholesterol, Ergocalciferol, UV-irradiation, Vitamin D2, |
| Online Access: | http://hdl.handle.net/10261/194266 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100003593 http://dx.doi.org/10.13039/100012818 http://dx.doi.org/10.13039/501100004837 |
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