An ultrahigh-performance liquid chromatography-time-of-flight mass spectrometry metabolomic approach to studying the impact of moderate red-wine consumption on urinary metabolome
Moderate red-wine consumption has been widely described to exert several benefits in human health. This is mainly due to its unique content of bioactive polyphenols, which suffer several modifications along their pass through the digestive system, including microbial transformation in the colon and phase-II metabolism, until they are finally excreted in urine and feces. To determine the impact of moderate wine consumption in the overall urinary metabolome of healthy volunteers (n = 41), samples from a red-wine interventional study (250 mL/day, 28 days) were investigated. Urine (24 h) was collected before and after intervention and analyzed by an untargeted ultrahigh-performance liquid chromatography-time-of-flight mass spectrometry metabolomics approach. 94 compounds linked to wine consumption, including specific wine components (tartaric acid), microbial-derived phenolic metabolites (5-(dihydroxyphenyl)-γ-valerolactones and 4-hydroxyl-5-(phenyl)-valeric acids), and endogenous compounds were identified. Also, some relationships between parallel fecal and urinary metabolomes are discussed.
Main Authors: | , , , , |
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Other Authors: | |
Format: | artículo biblioteca |
Published: |
American Chemical Society
2018
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Subjects: | Biomarkers, Polyphenols, Wine, Urinary metabolome, Phenolic microbial metabolites, UHPLC−TOF MS, |
Online Access: | http://hdl.handle.net/10261/192117 http://dx.doi.org/10.13039/100012818 http://dx.doi.org/10.13039/501100003339 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100010198 http://dx.doi.org/10.13039/501100011033 |
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