Antioxidant characterization and biological effects of grape pomace extracts supplementation in Caenorhabditis elegans

The aim of this work was to evaluate the biological activity of four grape pomace (GP) extracts that are rich in polyphenols using C. elegans as an in vivo model. Different concentrations of the GP extracts were assessed for their effects on the resistance of C. elegans against thermally induced oxidative stress, accumulation of reactive oxygen species (ROS), and lifespan. The cultivation of C. elegans with relatively low concentrations of GP extracts increased their resistance against thermal stress and prolonged their lifespan, while high levels displayed detrimental effects. In the studied extracts, maximum protection was observed for levels of polyphenols around 7 to 9 µg gallic acid equivalents per cultivation plate. The obtained results suggested that small changes in the ROS levels could have beneficial effects, although further studies are required to fully understand the impact of the extracts and assayed doses on ROS levels to explain the mechanism that is involved in the observed effects

Bibliographic Details

Main Authors:	Ayuda-Durán, Begoña, González-Manzano, Susana, Gil-Sánchez, Irene, Moreno-Arribas, M. Victoria, Bartolomé, Begoña, Sanz-Buenhombre, Marisa, Guadarrama, Alberto, Santos-Buelga, Celestino, González-Paramás, Ana M.
Other Authors:	Consejo Superior de Investigaciones Científicas (España)
Format:	artículo biblioteca
Published:	Multidisciplinary Digital Publishing Institute 2019-02-15
Subjects:	Grape pomace, C. elegans, Oxidative stress, ROS, Lifespan, Antioxidant activity,
Online Access:	http://hdl.handle.net/10261/177042 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100003339 http://dx.doi.org/10.13039/501100003176 http://dx.doi.org/10.13039/501100014180
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