Enzymatic extraction of pectin from artichoke (Cynara scolymus L.) by-products using Celluclast®1.5L
The aim of this study was to optimise pectin extraction from artichoke by-products with Celluclast1.5L using an experimental design analysed by response-surface methodology (RSM). The variables optimised were artichoke by-product powder concentration (2–7%, X), enzyme dose (2.2–13.3 U g, X) and extraction time (6–24 h, X). The variables studied were galacturonic acid (GalA) (R 93.9) and pectic neutral sugars (R 92.8) content and pectin yield (R 88.6). In the optimum extraction conditions (X = 6.5%; X = 10.1 U g; X = 27.2 h), pectin yield was 176 mgg dry matter (DM). Considering 27.2 h of treatment as the +α value given by the design, the extraction time was increased up to 48 h obtaining a yield of 221 mg g DM. The enzymatic method optimised allows obtaining artichoke pectin with good yield, high GalA (720 mg g DM) and arabinose (127.6mgg DM) contents and degree of methylation of 19.5%.
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Format: | artículo biblioteca |
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Elsevier
2018
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Subjects: | RSM, Celluclast, By-products, Artichoke, Pectin extraction, Cynara scolymus, |
Online Access: | http://hdl.handle.net/10261/170947 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100003176 http://dx.doi.org/10.13039/100012818 |
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dig-cial-es-10261-1709472020-12-13T09:21:58Z Enzymatic extraction of pectin from artichoke (Cynara scolymus L.) by-products using Celluclast®1.5L Sabater, Carlos Corzo, Nieves Olano, Agustín Montilla, Antonia Ministerio de Economía y Competitividad (España) Ministerio de Ciencia e Innovación (España) Ministerio de Educación, Cultura y Deporte (España) Comunidad de Madrid Danone Institute RSM Celluclast By-products Artichoke Pectin extraction Cynara scolymus The aim of this study was to optimise pectin extraction from artichoke by-products with Celluclast1.5L using an experimental design analysed by response-surface methodology (RSM). The variables optimised were artichoke by-product powder concentration (2–7%, X), enzyme dose (2.2–13.3 U g, X) and extraction time (6–24 h, X). The variables studied were galacturonic acid (GalA) (R 93.9) and pectic neutral sugars (R 92.8) content and pectin yield (R 88.6). In the optimum extraction conditions (X = 6.5%; X = 10.1 U g; X = 27.2 h), pectin yield was 176 mgg dry matter (DM). Considering 27.2 h of treatment as the +α value given by the design, the extraction time was increased up to 48 h obtaining a yield of 221 mg g DM. The enzymatic method optimised allows obtaining artichoke pectin with good yield, high GalA (720 mg g DM) and arabinose (127.6mgg DM) contents and degree of methylation of 19.5%. This work has been funded by MICINN of Spain, Project AGL2014-53445-R; ALIBIRD-CM S-2013/ABI-272, Comunidad Autónoma de Madrid and by the Spanish Danone Institute. Carlos Sabater thanks his FPU Predoc contract from Spanish MECD (FPU14/03619). Peer Reviewed 2018-10-11T10:06:24Z 2018-10-11T10:06:24Z 2018 2018-10-11T10:06:26Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.1016/j.carbpol.2018.02.055 issn: 0144-8617 e-issn: 1879-1344 Carbohydrate Polymers 190: 43-49 (2018) http://hdl.handle.net/10261/170947 10.1016/j.carbpol.2018.02.055 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100003176 http://dx.doi.org/10.13039/100012818 29628258 #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# S2013/ABI-2728/ALIBIRD info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2014-53445-R Postprint https://doi.org/10.1016/j.carbpol.2018.02.055 Sí open Elsevier |
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RSM Celluclast By-products Artichoke Pectin extraction Cynara scolymus RSM Celluclast By-products Artichoke Pectin extraction Cynara scolymus |
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RSM Celluclast By-products Artichoke Pectin extraction Cynara scolymus RSM Celluclast By-products Artichoke Pectin extraction Cynara scolymus Sabater, Carlos Corzo, Nieves Olano, Agustín Montilla, Antonia Enzymatic extraction of pectin from artichoke (Cynara scolymus L.) by-products using Celluclast®1.5L |
description |
The aim of this study was to optimise pectin extraction from artichoke by-products with Celluclast1.5L using an experimental design analysed by response-surface methodology (RSM). The variables optimised were artichoke by-product powder concentration (2–7%, X), enzyme dose (2.2–13.3 U g, X) and extraction time (6–24 h, X). The variables studied were galacturonic acid (GalA) (R 93.9) and pectic neutral sugars (R 92.8) content and pectin yield (R 88.6). In the optimum extraction conditions (X = 6.5%; X = 10.1 U g; X = 27.2 h), pectin yield was 176 mgg dry matter (DM). Considering 27.2 h of treatment as the +α value given by the design, the extraction time was increased up to 48 h obtaining a yield of 221 mg g DM. The enzymatic method optimised allows obtaining artichoke pectin with good yield, high GalA (720 mg g DM) and arabinose (127.6mgg DM) contents and degree of methylation of 19.5%. |
author2 |
Ministerio de Economía y Competitividad (España) |
author_facet |
Ministerio de Economía y Competitividad (España) Sabater, Carlos Corzo, Nieves Olano, Agustín Montilla, Antonia |
format |
artículo |
topic_facet |
RSM Celluclast By-products Artichoke Pectin extraction Cynara scolymus |
author |
Sabater, Carlos Corzo, Nieves Olano, Agustín Montilla, Antonia |
author_sort |
Sabater, Carlos |
title |
Enzymatic extraction of pectin from artichoke (Cynara scolymus L.) by-products using Celluclast®1.5L |
title_short |
Enzymatic extraction of pectin from artichoke (Cynara scolymus L.) by-products using Celluclast®1.5L |
title_full |
Enzymatic extraction of pectin from artichoke (Cynara scolymus L.) by-products using Celluclast®1.5L |
title_fullStr |
Enzymatic extraction of pectin from artichoke (Cynara scolymus L.) by-products using Celluclast®1.5L |
title_full_unstemmed |
Enzymatic extraction of pectin from artichoke (Cynara scolymus L.) by-products using Celluclast®1.5L |
title_sort |
enzymatic extraction of pectin from artichoke (cynara scolymus l.) by-products using celluclast®1.5l |
publisher |
Elsevier |
publishDate |
2018 |
url |
http://hdl.handle.net/10261/170947 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100003176 http://dx.doi.org/10.13039/100012818 |
work_keys_str_mv |
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_version_ |
1777671308337741824 |