Enzymatic extraction of pectin from artichoke (Cynara scolymus L.) by-products using Celluclast®1.5L
The aim of this study was to optimise pectin extraction from artichoke by-products with Celluclast1.5L using an experimental design analysed by response-surface methodology (RSM). The variables optimised were artichoke by-product powder concentration (2–7%, X), enzyme dose (2.2–13.3 U g, X) and extraction time (6–24 h, X). The variables studied were galacturonic acid (GalA) (R 93.9) and pectic neutral sugars (R 92.8) content and pectin yield (R 88.6). In the optimum extraction conditions (X = 6.5%; X = 10.1 U g; X = 27.2 h), pectin yield was 176 mgg dry matter (DM). Considering 27.2 h of treatment as the +α value given by the design, the extraction time was increased up to 48 h obtaining a yield of 221 mg g DM. The enzymatic method optimised allows obtaining artichoke pectin with good yield, high GalA (720 mg g DM) and arabinose (127.6mgg DM) contents and degree of methylation of 19.5%.
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | artículo biblioteca |
| Published: |
Elsevier
2018
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| Subjects: | RSM, Celluclast, By-products, Artichoke, Pectin extraction, Cynara scolymus, |
| Online Access: | http://hdl.handle.net/10261/170947 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100003176 http://dx.doi.org/10.13039/100012818 |
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