Foodomics study on the effects of extracellular production of hydrogen peroxide by rosemary polyphenols on the anti-proliferative activity of rosemary polyphenols against HT-29 cells

A number of studies have demonstrated a strong association between the antioxidant properties of rosemary polyphenols and their chemoprotective activity. However, the prooxidant effects of rosemary polyphenols have been rarely reported. In this work, a foodomics study is performed to investigate the in vitro autooxidation of carnosic acid (CA), carnosol (CS) and a polyphenol-enriched rosemary extract (SC-RE) in cell culture conditions. The results revealed that rosemary polyphenols autooxidation in culture medium generated H2O2 at different rates. Generated H2O2 levels by SC-RE and CA, but not CS, were correlated with intracellular reactive oxygen species (ROS) generation in HT-29 cells, and were partially involved in their anti-proliferative effect in this cell line. These compounds also induced different effects on glutathione metabolism. Results also indicated that high extracellular H2O2 concentrations, resulting of using high (45 μg/mL) SC-RE concentration in culture media, exerted some artifactual effects related with cell cycle, but they did not influence the expression of relevant molecular biomarkers of stress.

Bibliographic Details

Main Authors:	Valdés, Alberto, García-Cañas, Virginia, Simó, Carolina, Cifuentes, Alejandro
Other Authors:	The Scientific and Technological Research Council of Turkey
Format:	artículo biblioteca
Published:	Wiley-VCH 2016
Subjects:	Antioxidant, Reactive oxygen species, Prooxidant, Glutathione, Colon cancer,
Online Access:	http://hdl.handle.net/10261/150255 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/100012818
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