Release of antioxidant peptides from the body wall proteins of the sea cucumber Isostichopus fuscus
Proteases from the tentacles of the sea cucumber Isostichopus fuscus were partially purified and used to produce antioxidant peptides from body wall proteins of this marine species. Three proteins (105, 68, and 39 kDa) were identified by SDS-PAGE in the proteolytic extract of the tentacles. Protein hydrolyzates were generated with gelatin and crude protein substrates from body wall, and peptidic fractions lower and higher than 3 kDa were obtained to evaluate their oxygen radical scavenging capacity (ORAC). The 3 kDa-fraction obtained from the crude protein hydrolyzate showed the highest ORAC value (0.92 ± 0.04 μmol Trolox equivalent/mg protein). This fraction was selected to purify peptides potentially responsible for the activity that might be used as ingredients for development of functional foods.
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| Other Authors: | |
| Format: | artículo biblioteca |
| Published: |
Natural Product Communications
2015
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| Subjects: | Isostichopus fuscus, Antioxidant peptides, Tentacles, Sea cucumber, Proteases, |
| Online Access: | http://hdl.handle.net/10261/149948 http://dx.doi.org/10.13039/501100003141 http://dx.doi.org/10.13039/501100004173 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100003339 |
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