Quantitative determination of active Bowman-Birk isoinhibitors, IBB1 and IBBD2, in commercial soymilks
Naturally-occurring serine protease inhibitors of the Bowman-Birk family exert their potential chemopreventive and/or therapeutic properties via protease inhibition. In this study, we have quantified the amounts of active BBI isoinhibitors, IBB1 and IBBD2, in six commercial soymilks. By using cation exchange chromatography, the BBI isoinhibitors were isolated and their specific trypsin inhibitory activity was used to estimate their amounts in soymilk samples. IBB1 and IBBD2 concentrations ranged from 0.44 to 5.20 and 0.27 to 4.60 mg/100 ml of soymilk, respectively; total BBI, considered as the sum of both isoinhibitors, ranged from 0.60 to 9.07 mg/100 ml of soymilk. These data show that physiologically relevant amounts of active BBI are present in commercial soymilk and may exert potential health-promoting effects.
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Elsevier
2014
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| Subjects: | Kunitz, Chemoprevention, Protease inhibitors, Serine proteases, Soymilk, Bowman-Birk inhibitors, |
| Online Access: | http://hdl.handle.net/10261/113632 http://dx.doi.org/10.13039/501100007273 http://dx.doi.org/10.13039/501100002322 http://dx.doi.org/10.13039/501100003329 |
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dig-cial-es-10261-1136322018-09-19T12:11:42Z Quantitative determination of active Bowman-Birk isoinhibitors, IBB1 and IBBD2, in commercial soymilks Arques, María del Carmen Marín-Manzano, M. Carmen Brito da Rocha, L. Clarissa Hernández-Ledesma, Blanca Recio, Isidra Clemente, Alfonso Comisión Interministerial de Ciencia y Tecnología, CICYT (España) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil) Universidad de Córdoba (España) Ministerio de Economía y Competitividad (España) Kunitz Chemoprevention Protease inhibitors Serine proteases Soymilk Bowman-Birk inhibitors Naturally-occurring serine protease inhibitors of the Bowman-Birk family exert their potential chemopreventive and/or therapeutic properties via protease inhibition. In this study, we have quantified the amounts of active BBI isoinhibitors, IBB1 and IBBD2, in six commercial soymilks. By using cation exchange chromatography, the BBI isoinhibitors were isolated and their specific trypsin inhibitory activity was used to estimate their amounts in soymilk samples. IBB1 and IBBD2 concentrations ranged from 0.44 to 5.20 and 0.27 to 4.60 mg/100 ml of soymilk, respectively; total BBI, considered as the sum of both isoinhibitors, ranged from 0.60 to 9.07 mg/100 ml of soymilk. These data show that physiologically relevant amounts of active BBI are present in commercial soymilk and may exert potential health-promoting effects. A.C. acknowledges support by ERDF-co-financed Grant from the Spanish CICYT (AGL2011-26353). A.C. is involved in COST Action FA1005 INFOGEST on Food Digestion. L.C.B.R. acknowledges support from CAPES (Programa Institucional de Doutorado Sanduíche no Exterior–PDSE process number 9004-11-4), Brazil. We are grateful to the proteomics Facility of University of Cordoba (Spain) for carrying out peptide mass fingerprinting. B.H.-L. thanks Ministry of Economy and Competitiveness for her “Ramón y Cajal” contract. Peer Reviewed 2015-04-14T09:30:09Z 2015-04-14T09:30:09Z 2014 2015-04-14T09:30:09Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.1016/j.foodchem.2014.01.024 issn: 0308-8146 Food Chemistry 155: 24-30 (2014) http://hdl.handle.net/10261/113632 10.1016/j.foodchem.2014.01.024 http://dx.doi.org/10.13039/501100007273 http://dx.doi.org/10.13039/501100002322 http://dx.doi.org/10.13039/501100003329 Postprint http://dx.doi.org/10.1016/j.foodchem.2014.01.024 Sí open Elsevier |
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Kunitz Chemoprevention Protease inhibitors Serine proteases Soymilk Bowman-Birk inhibitors Kunitz Chemoprevention Protease inhibitors Serine proteases Soymilk Bowman-Birk inhibitors |
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Kunitz Chemoprevention Protease inhibitors Serine proteases Soymilk Bowman-Birk inhibitors Kunitz Chemoprevention Protease inhibitors Serine proteases Soymilk Bowman-Birk inhibitors Arques, María del Carmen Marín-Manzano, M. Carmen Brito da Rocha, L. Clarissa Hernández-Ledesma, Blanca Recio, Isidra Clemente, Alfonso Quantitative determination of active Bowman-Birk isoinhibitors, IBB1 and IBBD2, in commercial soymilks |
| description |
Naturally-occurring serine protease inhibitors of the Bowman-Birk family exert their potential chemopreventive and/or therapeutic properties via protease inhibition. In this study, we have quantified the amounts of active BBI isoinhibitors, IBB1 and IBBD2, in six commercial soymilks. By using cation exchange chromatography, the BBI isoinhibitors were isolated and their specific trypsin inhibitory activity was used to estimate their amounts in soymilk samples. IBB1 and IBBD2 concentrations ranged from 0.44 to 5.20 and 0.27 to 4.60 mg/100 ml of soymilk, respectively; total BBI, considered as the sum of both isoinhibitors, ranged from 0.60 to 9.07 mg/100 ml of soymilk. These data show that physiologically relevant amounts of active BBI are present in commercial soymilk and may exert potential health-promoting effects. |
| author2 |
Comisión Interministerial de Ciencia y Tecnología, CICYT (España) |
| author_facet |
Comisión Interministerial de Ciencia y Tecnología, CICYT (España) Arques, María del Carmen Marín-Manzano, M. Carmen Brito da Rocha, L. Clarissa Hernández-Ledesma, Blanca Recio, Isidra Clemente, Alfonso |
| format |
artículo |
| topic_facet |
Kunitz Chemoprevention Protease inhibitors Serine proteases Soymilk Bowman-Birk inhibitors |
| author |
Arques, María del Carmen Marín-Manzano, M. Carmen Brito da Rocha, L. Clarissa Hernández-Ledesma, Blanca Recio, Isidra Clemente, Alfonso |
| author_sort |
Arques, María del Carmen |
| title |
Quantitative determination of active Bowman-Birk isoinhibitors, IBB1 and IBBD2, in commercial soymilks |
| title_short |
Quantitative determination of active Bowman-Birk isoinhibitors, IBB1 and IBBD2, in commercial soymilks |
| title_full |
Quantitative determination of active Bowman-Birk isoinhibitors, IBB1 and IBBD2, in commercial soymilks |
| title_fullStr |
Quantitative determination of active Bowman-Birk isoinhibitors, IBB1 and IBBD2, in commercial soymilks |
| title_full_unstemmed |
Quantitative determination of active Bowman-Birk isoinhibitors, IBB1 and IBBD2, in commercial soymilks |
| title_sort |
quantitative determination of active bowman-birk isoinhibitors, ibb1 and ibbd2, in commercial soymilks |
| publisher |
Elsevier |
| publishDate |
2014 |
| url |
http://hdl.handle.net/10261/113632 http://dx.doi.org/10.13039/501100007273 http://dx.doi.org/10.13039/501100002322 http://dx.doi.org/10.13039/501100003329 |
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