Quantitative determination of active Bowman-Birk isoinhibitors, IBB1 and IBBD2, in commercial soymilks

Naturally-occurring serine protease inhibitors of the Bowman-Birk family exert their potential chemopreventive and/or therapeutic properties via protease inhibition. In this study, we have quantified the amounts of active BBI isoinhibitors, IBB1 and IBBD2, in six commercial soymilks. By using cation exchange chromatography, the BBI isoinhibitors were isolated and their specific trypsin inhibitory activity was used to estimate their amounts in soymilk samples. IBB1 and IBBD2 concentrations ranged from 0.44 to 5.20 and 0.27 to 4.60 mg/100 ml of soymilk, respectively; total BBI, considered as the sum of both isoinhibitors, ranged from 0.60 to 9.07 mg/100 ml of soymilk. These data show that physiologically relevant amounts of active BBI are present in commercial soymilk and may exert potential health-promoting effects.

Bibliographic Details

Main Authors:	Arques, María del Carmen, Marín-Manzano, M. Carmen, Brito da Rocha, L. Clarissa, Hernández-Ledesma, Blanca, Recio, Isidra, Clemente, Alfonso
Other Authors:	Comisión Interministerial de Ciencia y Tecnología, CICYT (España)
Format:	artículo biblioteca
Published:	Elsevier 2014
Subjects:	Kunitz, Chemoprevention, Protease inhibitors, Serine proteases, Soymilk, Bowman-Birk inhibitors,
Online Access:	http://hdl.handle.net/10261/113632 http://dx.doi.org/10.13039/501100007273 http://dx.doi.org/10.13039/501100002322 http://dx.doi.org/10.13039/501100003329
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