In vitro fermentation of lactulose-derived oligosaccharides by mixed fecal microbiota
Fermentation properties of oligosaccharides derived from lactulose (OsLu) and lactose (GOS) have been assessed in pH-controlled anaerobic batch cultures using lactulose and Vivinal-GOS as reference carbohydrates. Changes in gut bacterial populations and their metabolic activities were monitored over 24 h by fluorescent in situ hybridization (FISH) and by measurement of short-chain fatty acid (SCFA) production. Lactulose-derived oligosaccharides were selectively fermented by Bifidobacterium and lactic acid bacterial populations producing higher SCFA concentrations compared to GOS. The highest total SCFA production was from Vivinal-GOS > lactulose > OsLu > GOS. Longer incubation periods produced a selective fermentation of OsLu when they were used as a carbon source reaching the highest selective index scores. The new oligosaccharides may constitute a good alternative to lactulose, and they could belong to a new generation of prebiotics to be used as a functional ingredient for improving the composition of gut microflora. © 2012 American Chemical Society.
| Main Authors: | , , , , , , |
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| Other Authors: | |
| Format: | artículo biblioteca |
| Published: |
American Chemical Society
2012
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| Subjects: | Lactulose, Ooligosaccharides, Short-chain fatty acids, Gut microbiota, Fermentation, |
| Online Access: | http://hdl.handle.net/10261/101356 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/100012818 http://dx.doi.org/10.13039/501100011698 |
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