A Foodomics approach: CE-MS for comparative metabolomics of colon cancer cells treated with dietary polyphenols
The potential of capillary electrophoresis-mass spectrometry (CE-MS) for metabolomics is demonstrated through the analysis of metabolites from human HT29 colon cancer cells treated and non-treated with dietary polyphenols. Prior to CE-MS analysis, four different metabolite purification strategies are investigated. Namely, the results obtained after methanol deproteinization, ultrafiltration, and two solid-phase extraction methods using C18 and polymer-based cartridges are described. These generic methods can have broad applications to analyze metabolites in a large variety of matrices and fields, including the new Foodomics area.
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| Main Authors: | , , , |
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| Other Authors: | |
| Format: | capítulo de libro biblioteca |
| Published: |
Springer
2012
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| Subjects: | Colon cancer, Metabolomics, Dietary polyphenols, CE, CE-MS, Foodomics, |
| Online Access: | http://hdl.handle.net/10261/101222 http://dx.doi.org/10.13039/501100004837 |
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