Separation and characterization of phlorotannins from brown algae Cystoseira abies-marina by comprehensive two-dimensional liquid chromatography
Phlorotannins are an important class of polyphenolic compounds only found in brown algae. The chemical analysis of these bioactive polyphenols is rather difficult due to the great chemical variability and complexity of the natural composition of these components in algae, forming large phloroglucinol polymers. In the present work, a new approach based on the use of comprehensive 2D LC (LC × LC) is shown to analyze this complex family of compounds. The developed LC × LC methodology is based on the coupling of a hydrophilic-interaction LC (HILIC)-based separation in the first dimension and an RP-based separation in the second dimension. The employment of this online coupling together with DAD and MS/MS allowed the separation and identification of more than 50 compounds in a Cystoseira abies-marina brown alga extract. Phlorotannins containing from 5 to 17 phloroglucinol units were identified in this sample by HILIC × RP-DAD-MS/MS. Besides, using the 2,4-dimethoxybenzaldehyde assay, it was possible to determine that the total amount of phlorotannins present in the extract was 40.2 mg phloroglucinol equivalents per gram of extract. To our knowledge, this work is the first demonstration of the usefulness of HILIC × RP-DAD-MS/MS for the determination of phlorotannins. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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Wiley-VCH
2014
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Subjects: | Phlorotannins, Brown algae, Cystoseira abies-marina, LC × LC, Polymeric polyphenols, |
Online Access: | http://hdl.handle.net/10261/100287 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/100012818 |
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dig-cial-es-10261-1002872018-09-12T11:14:02Z Separation and characterization of phlorotannins from brown algae Cystoseira abies-marina by comprehensive two-dimensional liquid chromatography Montero, Lidia Herrero, Miguel Ibáñez, Elena Cifuentes, Alejandro Comunidad de Madrid Ministerio de Ciencia e Innovación (España) Phlorotannins Brown algae Cystoseira abies-marina LC × LC Polymeric polyphenols Phlorotannins are an important class of polyphenolic compounds only found in brown algae. The chemical analysis of these bioactive polyphenols is rather difficult due to the great chemical variability and complexity of the natural composition of these components in algae, forming large phloroglucinol polymers. In the present work, a new approach based on the use of comprehensive 2D LC (LC × LC) is shown to analyze this complex family of compounds. The developed LC × LC methodology is based on the coupling of a hydrophilic-interaction LC (HILIC)-based separation in the first dimension and an RP-based separation in the second dimension. The employment of this online coupling together with DAD and MS/MS allowed the separation and identification of more than 50 compounds in a Cystoseira abies-marina brown alga extract. Phlorotannins containing from 5 to 17 phloroglucinol units were identified in this sample by HILIC × RP-DAD-MS/MS. Besides, using the 2,4-dimethoxybenzaldehyde assay, it was possible to determine that the total amount of phlorotannins present in the extract was 40.2 mg phloroglucinol equivalents per gram of extract. To our knowledge, this work is the first demonstration of the usefulness of HILIC × RP-DAD-MS/MS for the determination of phlorotannins. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Funded by: BEA (Banco Español de Algas, Las Palmas de Gran Canaria, Spain). Grant Number: AGL2011-29857-C03-01 and ALIBIRD. Grant Number: S2009/AGR-1469. Peer Reviewed 2014-07-21T12:51:11Z 2014-07-21T12:51:11Z 2014 2014-07-21T12:51:12Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.1002/elps.201400133 issn: 0173-0835 e-issn: 1522-2683 Electrophoresis 35(11): 1644-1651 (2014) http://hdl.handle.net/10261/100287 10.1002/elps.201400133 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/100012818 #PLACEHOLDER_PARENT_METADATA_VALUE# S2009/AGR-1469/ALIBIRD Postprint htpp://dx.doi.org/10.1002/elps.201400133 Sí open Wiley-VCH |
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Phlorotannins Brown algae Cystoseira abies-marina LC × LC Polymeric polyphenols Phlorotannins Brown algae Cystoseira abies-marina LC × LC Polymeric polyphenols |
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Phlorotannins Brown algae Cystoseira abies-marina LC × LC Polymeric polyphenols Phlorotannins Brown algae Cystoseira abies-marina LC × LC Polymeric polyphenols Montero, Lidia Herrero, Miguel Ibáñez, Elena Cifuentes, Alejandro Separation and characterization of phlorotannins from brown algae Cystoseira abies-marina by comprehensive two-dimensional liquid chromatography |
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Phlorotannins are an important class of polyphenolic compounds only found in brown algae. The chemical analysis of these bioactive polyphenols is rather difficult due to the great chemical variability and complexity of the natural composition of these components in algae, forming large phloroglucinol polymers. In the present work, a new approach based on the use of comprehensive 2D LC (LC × LC) is shown to analyze this complex family of compounds. The developed LC × LC methodology is based on the coupling of a hydrophilic-interaction LC (HILIC)-based separation in the first dimension and an RP-based separation in the second dimension. The employment of this online coupling together with DAD and MS/MS allowed the separation and identification of more than 50 compounds in a Cystoseira abies-marina brown alga extract. Phlorotannins containing from 5 to 17 phloroglucinol units were identified in this sample by HILIC × RP-DAD-MS/MS. Besides, using the 2,4-dimethoxybenzaldehyde assay, it was possible to determine that the total amount of phlorotannins present in the extract was 40.2 mg phloroglucinol equivalents per gram of extract. To our knowledge, this work is the first demonstration of the usefulness of HILIC × RP-DAD-MS/MS for the determination of phlorotannins. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
author2 |
Comunidad de Madrid |
author_facet |
Comunidad de Madrid Montero, Lidia Herrero, Miguel Ibáñez, Elena Cifuentes, Alejandro |
format |
artículo |
topic_facet |
Phlorotannins Brown algae Cystoseira abies-marina LC × LC Polymeric polyphenols |
author |
Montero, Lidia Herrero, Miguel Ibáñez, Elena Cifuentes, Alejandro |
author_sort |
Montero, Lidia |
title |
Separation and characterization of phlorotannins from brown algae Cystoseira abies-marina by comprehensive two-dimensional liquid chromatography |
title_short |
Separation and characterization of phlorotannins from brown algae Cystoseira abies-marina by comprehensive two-dimensional liquid chromatography |
title_full |
Separation and characterization of phlorotannins from brown algae Cystoseira abies-marina by comprehensive two-dimensional liquid chromatography |
title_fullStr |
Separation and characterization of phlorotannins from brown algae Cystoseira abies-marina by comprehensive two-dimensional liquid chromatography |
title_full_unstemmed |
Separation and characterization of phlorotannins from brown algae Cystoseira abies-marina by comprehensive two-dimensional liquid chromatography |
title_sort |
separation and characterization of phlorotannins from brown algae cystoseira abies-marina by comprehensive two-dimensional liquid chromatography |
publisher |
Wiley-VCH |
publishDate |
2014 |
url |
http://hdl.handle.net/10261/100287 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/100012818 |
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