Release of multifunctional peptides by gastrointestinal digestion of sea cucumber (Isostichopus badionotus)
Sea cucumber is a benthic marine organism distributed worldwide and used as food in several Asian countries. The species Isostichopus badionotus is captured intensively off the Yucatan Peninsula, Mexico. Boiled I. badionotus was subjected to in vitro simulated gastrointestinal digestion using pepsin and a pepsin-Corolase PP® mixture. ACE-inhibitory and radical scavenging activities, iron reducing capacity and cytotoxic effects against colorectal cancer cells were evaluated in the hydrolysates and their ultrafiltered fractions. ACE-inhibitory activity was potent in fractions containing peptides <3000Da, an effect augmented with combined action of gastric (pepsin) and intestinal (Corolase PP®) enzymes (IC50=0.038±0.004mg/mL). Antioxidant activity was exerted by peptides with low and high molecular weights, depending on hydrolysis method. This is the first report of cytotoxic capacity against colorectal HT-29 cells in peptides from sea cucumber. Sea cucumber hydrolysates and ultrafiltered fractions are potential ingredients for development of functional foods. © 2013 Elsevier Ltd.
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| Format: | artículo biblioteca |
| Published: |
Elsevier
2013
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| Subjects: | Simulated gastrointestinal digestion, Isostichopus badionotus, Sea cucumber, Biological activities, Bioactive peptides, |
| Online Access: | http://hdl.handle.net/10261/100265 http://dx.doi.org/10.13039/501100003339 http://dx.doi.org/10.13039/501100000780 |
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