In vitro bifidogenic effect of Maillard-type milk protein-galactose conjugates on the human intestinal microbiota

This work addresses the effect of sodium caseinate (SC) and β-lactoglobulin (β-Lg) glycosylated with galactose (Gal) via the Maillard reaction on the human intestinal microbiota, using small-scale in vitro batch cultures inoculated with human faeces. The glycation process was controlled to maximise formation of the corresponding Amadori compound, tagatosyl-lysine, a derivative of tagatose. Both glycoconjugates (SC:Gal and β-Lg:Gal) induced a significant increase in bifidobacteria, whilst Lactobacillus-Enterococcus, Clostridium histolyticum, Atopobium and Bacteroides populations were unmodified. Additionally, β-Lg:Gal glycoconjugate also significantly stimulated the growth of the Clostridium coccoides-Eubacterium rectal group. SC:Gal glycoconjugate gave rise to a significant increase in acetic acid concentration. From these results it could be inferred that the conjugation of proteins or peptides with non-prebiotic carbohydrates, such as Gal, might lead to new prebiotic products with different properties than the free carbohydrate or native protein. © 2013 Elsevier Ltd.

Saved in:
Bibliographic Details
Main Authors: Corzo-Martínez, Marta, Hernández-Hernández, Oswaldo, Villamiel, Mar, Rastall, Robert A., Moreno, F. Javier
Other Authors: Ministerio de Economía y Competitividad (España)
Format: artículo biblioteca
Published: Elsevier 2013
Online Access:http://hdl.handle.net/10261/100098
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/501100003339
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100011698
Tags: Add Tag
No Tags, Be the first to tag this record!