Recombinant Mycoplasma mycoides proteins elicit protective immune responses against contagious bovine pleuropneumonia

Mycoplasma mycoides subsp. mycoides (Mmm) is the causative agent of contagious bovine pleuropneumonia (CBPP), a devastating respiratory disease mainly affecting cattle in sub-Saharan Africa. The current vaccines are based on live-attenuated Mmmstrains and present problems with temperature stability, duration of immunity and adverse reactions, thus new vaccines are needed to overcome these issues. We used a reverse vaccinology approach to identify 66 Mmm potential vaccine candidates. The selection and grouping of the antigens was based on the presence of specific antibodies in sera from CBPP-positive animals. The antigens were used to immunize male Boran cattle (Bos indicus) followed by a challenge with the Mmm strain Afadé. Two of the groups immunized with five proteins each showed protection after the Mmm challenge (Groups A and C; P < 0.05) and in one group (Group C) Mmm could not be cultured from lung specimens. A third group (Group N) showed a reduced number of animals with lesions and the cultures for Mmm were also negative. While immunization with some of the antigens conferred protection, others may have increased immune-related pathology. This is the first report that Mmm recombinant proteins have been successfully used to formulate a prototype vaccine and these results pave the way for the development of a novel commercial vaccine.

Bibliographic Details

Main Authors:	Nkando, I., Pérez Casal, J., Mwirigi, M., Prysliak, T., Townsend, H., Berberov, E., Kuria, J., Mugambi, J., Soi, R., Liljander, Anne M., Jores, Joerg, Gerdts, V., Potter, A., Naessens, Jan, Wesonga, H.O.
Format:	Journal Article biblioteca
Language:	English
Published:	Elsevier 2016-03
Subjects:	animal diseases, disease control,
Online Access:	https://hdl.handle.net/10568/72741 https://doi.org/10.1016/j.vetimm.2016.02.010
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