Novel baculovirus-derived p67 subunit vaccines efficacious against East Coast fever in cattle

Two novel baculovirus-derived recombinant Theileria parva p67 constructs were tested for their vaccine potential against East Coast fever. Boran calves were immunized with a his-GFP-p67 fusion protein (GFP:p67DeltaSS) or with GP64:p67C, a protein fusion between a C-terminal domain of p67 and the baculovirus envelope protein GP64. Both GFP:p67DeltaSS and GP64:p67C induced antibodies with high ELISA titers that neutralized T. parva sporozoites with high efficiency. Upon challenge, a correlation was observed between the in vitro neutralizing capacity and the reduction in severe ECF for individual animals. A protection level upto 85% was obtained. This level of protection was achieved with only two inoculations of 100mug per dose, which is a major improvement over previous recombinant p67 products.

Bibliographic Details

Main Authors:	Kaba, S.A., Musoke, A.J., Schaap, D., Schetters, T., Rowlands, G.J., Vermeulen, A.N., Nene, Vishvanath, Vlak, J.M., Oers, M.M. van
Format:	Journal Article biblioteca
Language:	English
Published:	Elsevier 2005-04
Subjects:	cattle, east coast fever, baculoviridae [invertebrate viruses], theileria parva, vaccines, elisa, antibodies, sporozoa,
Online Access:	https://hdl.handle.net/10568/33217 https://doi.org/10.1016/j.vaccine.2004.10.043
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