A highly sensitive, non-radioactive assay for T cell activation in cattle: Applications in screening for antigens recognised by CD4+ and CD8+ T cells
We describe a highly sensitive, non-radioactive assay for T cell activation, based on the rapid induction of class II MHC expression by constitutively negative bovine endothelial cells, when cultured in the presence of supernatants derived from activated bovine T cells. We demonstrate the effectiveness of this assay in detecting rBoIFN y and activation of immune CD4 - and CD8 - T cell lines and clones in response to specific antigen and transfected COS-7 cells, respectively. We also demonstrate its utility in identifying purified pathogen fractions that activate immune CD4-T cell clones.
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| Main Authors: | , , , , , |
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| Format: | Journal Article biblioteca |
| Language: | English |
| Published: |
Elsevier
2000-05
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| Subjects: | cattle, antigens, bovidae, cells, antibodies, theileria parva, |
| Online Access: | https://hdl.handle.net/10568/33010 https://doi.org/10.1016/S0022-1759(00)00187-3 |
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