Standardization of a molecular diagnostic method for Cucumber mosaic virus (cmv) in Ecuadorian bananas
Several pests and diseases affect banana crop in Ecuador and Cucumber mosaic virus (cmv) is one of the most important pathogens. The aim of this research was to standardize a new molecular approach to achieve a sensitive and highly specific detection of cmv in Ecuadorian bananas. Specific primers were designed from the sequence encoding for the virus capsid protein. PC-F1, PC-R D1 and K-F primers, obtained from cDNA replicated from R NA of infected banana, allowed accurate virus detection by Reverse transcription and Hemi-nested PCR. Virus detection was possible even in asymptomatic plants, providing a tech-nology with potential use for the Ecuadorian banana producers.
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| Main Authors: | , |
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| Format: | info:eu-repo/semantics/article biblioteca |
| Language: | spa |
| Published: |
Corporación Colombiana de Investigación Agropecuaria (Agrosavia)
2016
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| Online Access: | http://revistacta.agrosavia.co/index.php/revista/article/view/562 http://hdl.handle.net/20.500.12324/35237 |
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