The embryonic and larvae developments of Tomato Anemonefish (Amphiprion frenatus Brevoort, 1856) in Khanh Hoa coast
Tomato Anemonefish (Amphiprion frenatus) embryo developed normally in seawater environment with incubated temperature 27 1C; salinity 34 - 35ppt; pH range from 8.1 to 8.3; O2 > 5mg/l; NH3 < 0.1mg/l and NO2 < 0.05mg/l during August/2000 to December/2000. The eggs are capsule shaped with the length of 2.68 0.02mm and the width of 0.97 0.37mm. Twelve hours after spawning, the embryo has entered the blastula. On the second day after spawning, the embryo is undergoing gastrula. After 40 hours, the heart is apparently and beats at a rate of 78 times per minute, iris can be visible. On the fifth day, the pectoral fins make their first appearance and the dorsal and ventral fin folds are well defined. On the eighth day, gills are more readily apparent, vestiges of the gill rakers are visible, mouth can be distinguished, anus can be seen. The tenth day, the yolk is absorbefacient, the embryonic mouth opens, larvae could eat immediately after hatching out. The larvae was 4.6 0.2mm in length. Brachionus plicatilis density of 5 inds/ml and Nannochloropsis oculata density of 106 inds/ml were added in 5 first days. After that, Artemia density of 5 inds/ml was instead of them and after one month, fish fed on fresh meat. Average actual fecundity of Amphiprion frenatus was 815 eggs. The interval of spawned batches prolonged from 10 to 14 days. Average hatching rate of each spawned batch was 82.09%. Average survival rate of one-month old fishes was 69.32% and survival rate of three months old fishes almost was 100%.
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| Format: | Journal Contribution biblioteca |
| Language: | English |
| Published: |
2002
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| Subjects: | Tomato anemonefish, Amphiprion frenatus, Embryonic development, Larvae development, |
| Online Access: | http://hdl.handle.net/1834/9270 |
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