Identification genetic characterization and population of Barbus brachycephalic caspius, Lucioperca lucioperca, Rutilus rutilus caspius, Rutilus frisii kutum and Salmo trutta caspius in southern part of the Caspian Sea by molecular method (Microsatellites) and formation DNA bank
In this study genetic characterization of Barbus brachycephalus caspius, Lucioperca lucioperca, Rutilus rutilus caspius, Rutilus frisi kutum and Salmo trutta caspius were examined by 611 samples from regions in East (Guilan province), Middle (Mazandaran province) and west (Golestan province) of southen part of the Caspian Sea. DNA was extracted from fin tissue by phenol -chlorophorm method and then PCR was performed using special primers. Statistical analysis of data was performed by Gene Alex, MEGA and Arlequin softwares. -Rutilus frisi kutum: The results showed that nine of ten primers were polymorphic loci. The mean of effective and observed alleles were 7.26±0.49 and 4.37±0.35 respectively. Also, the mean of expected and observed heterozygosity were 0.55±0.03 and 0.69±0.02 respectively Of the analysed loci, all of the samples (except Tajan and samples in LOC4 and Gilan samples in MFW2) possible tests were found to deviate significantly from the Hardy–Weinberg equilibrium (P<0.05). The genetic diversity was significantly different between samples of Golestan and Gilan, Golestan and sefidrood, Golestan and Tajan, Mazandaran and sefidrood and Gilan and Tajan (p<0.05). -Rutilus rutilus caspius: Sevan variable microsatellite loci were used to investigate genetic diversity and population structure of R. rutilus caspius. The mean of effective and observed alleles were 5.75±0.30 and 4.76±0.25 respectively. Also, the mean of expected and observed heterozygosity were 0.58±0.18 and 0.73±0.01 respectively. All of the samples (except golestan samples in LOC3) possible tests were found to deviate significantly from the Hardy–Weinberg equilibrium (P<0.05) Of the analysed loci, the genetic divergence was significantly different between samples of Golestan and Gilan, Gilan and Mazandaran and Gilan with Gorgan bay (p<0.05). -Salmo trutta caspius: Genetic characterization of S. trutta caspius was comparatively analyzed with mitochondrial DNA sequencing that 45 haplotypes was observed. The average of expected and observed heterozygosity were 0.61±0.35 and 0.33±0.12 respectively. The maximum of haplotype diversity (0.089±0.04) was in sardabrood river and the minimum was in Astara river (0.81±0.02). Also, the maximum of nucleotid diversity was 0.13±0.07 in Sardabrood and Chalos rivers and the minimum was 0.11±0.06 in Tonekabon river. In addition, the maximum and minimum of FST was 0.08 and 0.01 respectively. Of the analysed loci, the genetic divergence was significantly different between samples of Astara and Chalos, Astara and Tonekabon, Chalos and Karganrood and Tonekabon with Kaganrood (p<0.05). - Barbus brachycephalus caspius: The size of amplified fragment was 800 bp in all of the samples. There were 24 variable loci and 12 haplotype that the maximum of haplotype was in Gilan area (8 haplotype). The average of expected and observed heterozygosity were 0.003±0.35 and 0.42±0.12 respectively. The results showed that the haplotype diversity was significantly different between samples of Sefidrood whit other samples (p<0.05). In addition The maximum of nocleotid diversity was 0.005±0.003 in Sefidrood and minimum was 0.001± 0.001 in Tajan river. Of the analysed loci, the genetic divergence was significantly different between samples of Gilan and Tajan, Mazandaran and Sefidrood (p<0.05). -Lucioperca lucioperca: The genetic diversity of L. lucioperca was analyzed by using microsatellite markers. Seven primer sequences available for were tested to amplify microsatellite loci that all of loci were polymorphic. The mean of effective and observed alleles were 6.14±0.45 and 3.88±0.34 respectively. Also, the mean of expected and observed heterozygosity were 0.662±0.03 and 0.70±0.02 respectively. The most of samples in PflaL6, PflaL7and PflaL8 loci possible tests were found to deviate significantly from the Hardy–Weinberg equilibrium (P<0.05). The maximum of FST was 0.30 between Gilan and Mazandaran samples that there were minimum gene flow (8.18). The genetic divergence was significantly different between samples of Gilan and Mazandaran and Golestan whit mazandaran (p<0.05).
| Main Authors: | , , , , , , , , , |
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| Format: | monograph biblioteca |
| Language: | Persian |
| Published: |
Iranian Fisheries Science Research Institute
2016
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| Subjects: | Biology, Iran, Caspian Sea, Genetic variation, Barbus brachycephalus caspius, Lucioperca lucioperca, Rutilus rutilus caspius, Rutilus frisi kutum, Salmo trutta caspius, Identification, Population, Genetic, DNA, DNA bank, Molecular, Microsatellites, |
| Online Access: | http://hdl.handle.net/1834/40102 |
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