Population genetic structure of 2 species from Caspian Sea (Rutilus frissi kutum and Rutilus rutilus) and 2 species from Persian Gulf and Oman Sea (Rachycentron canadum and Eleutheronema tetradactylum) using Molecular Marker
Population structure of 2 species in the Caspian Sea (Rutilus frissi kutum and Rutilus rutilus) and 2 species in the Persian Gulf and Oman sea (Rachycentron canadum and Polynemus Plebeius) were studid by using Microsatellite and RAPD. For this 210 specimens of kutum (Khoshkrud, Tonekabon, Gorganrud, Anzali Lagoon and Kura River mouth), 90 (Gorgan Bay, Anzali Lagoon and Volga), 184 of cobia (Bushehr, daier, Bandarabass, bazm, lengeh) and 235 of (Khozestan, Bushehr, Bandarabass and chabahar) were sampled. Genomic DNA was extracted of fin using the phenol-chloroform .The quantity and quality of DNA from samples were assessed by spectrophptometer and 1% agarose gel electro-phoresis. PCR was carried out using microsatellite and RAPD primers. PCR products were separated on 8% polyacrylamide gels that were stained using silver nitrate. Result showed that in kutum the average of expected and observed heterozygosity was 0.54 and 0.49 respectively. Significant deviations from Hardy-Weinberg expectations were observed almost in all of location (P≤0.01, (P≤0.05). Using Fst and Rst there was significant difference between locations (P≤0.01). In study of Rutilus rutiluscaspicu the average of expected and observed heterozygosity was 0.5 and 0.7 respectively. Fst didn t show significant difference between iranian locations (P≥ 0.01) but it was Significant between Iranian populations and Russian populations (P≤ 0.05). In cobia the average of expected and observed heterozygosity was 0.655 and 0.874 respectively. Significant deviations from Hardy-Weinberg expectations were observed almost in all of location (P ≤0.01, (P≤ 0.05)). Significant differences (P<0.01) weren't observed between Rst recorded in the same region specimens studied but were observed between Rst recorded in the different region specimens studied. In Polynemus Plebeius, the observed heterozygosity in the Bushehr and Bandar abbas (0.22±0.13) was the highest and in Chabahar the lowest (0.171±0.175).Key word: Population Genetic, Rutilus frissi kutum ,Rutilus rutilus, Caspian Sea, Rachycentron canadum , Polynemus Plebeius, Persian Gulf and Oman sea.
| Main Authors: | , , |
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| Format: | monograph biblioteca |
| Language: | Persian |
| Published: |
Iranian Fisheries Science Research Institute
2011
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| Subjects: | Biology, Iran, Caspian Sea, Persian Gulf, Oman Sea, Anzali Lagoon, Gorgan Bay, Bushehr, Lengeh, Khouzestan, Chabahar, Population, Genetic, Rutilus frisii kutum, Rutilus rutilus, Rachycentron canadum, Polynemus Plebeius, Species, Eleutheronema tetradactylum, Molecular Marker, Specimens, Phenol-chloroform, DNA, |
| Online Access: | http://hdl.handle.net/1834/39648 |
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