Isolation, identification and cloning of growth hormone gene from Epinephlus coioides in E. coli
According to the role of the growth hormone gene in the aquaculture, in this research cloning growth hormone coioides (Epinephlus coioides) was evaluated in vector pTZ57R/T. After PCR product purification by kit QIA quick Gel Extraction, growth hormone gene was integrated into pTZ57R/T vector. Constructed vector was transferred in E. coli strain DH5α competent cells. The white colonies was recombinant bacteria, from that that plasmid extraction was performed. Cloning obtained conformed using with direct PCR and sequencing methods. Growth hormone cDNA from E. coioide has an open reading frame of 615 nucleotides and 204 amino acids respectively. The calculated molecular weight and predicted of isoelectric point of the growth hormone protein were 014/23 kDa and 9.6 respectively. The results of this study showed that growth hormone gene in pTZ57R/T vector successfully had been cloned and can be used to growth hormone gene expression and protein production. The operation of gene sequencing with growth hormone gene from E. coioides in the Gen Bank was showed similarity between them.
| Main Authors: | , , , , |
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| Format: | article biblioteca |
| Language: | Persian |
| Published: |
2016
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| Subjects: | Biology, Iran, Growth, Hormone, Cloning, Epinephelus coioides, pTZ57R/T vect, E. coli, Identification, Aquaculture, Gene, GenBank, |
| Online Access: | http://hdl.handle.net/1834/39381 |
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