Genetic status of tilapia at Badore landing site using RAPD markers
Molecular technique based on Random Amplified Polymorphic DNA (RAPD) analysis was applied to study genetic status among tilapia species from Badore landing site of Lekki lagoon. Individual variations within species population were assessed using PCR-RAP analysis with five Operon primers (OPC04, OPA02, OPB08, OPE02 and OPF03, Operon Technologies Inc, USA) which revealed dif ferent banding patterns of varying primer reproducibility. Graphical representation using UPGMA cluster analysis produced a dendrogram chart with five clusters (~f, ~e, ~p ,~S, and ~W) indicating different degrees of variations and similarities. There were various levels of genetic similarity observed possibly due to hybridization. Nevertheless, few distinct variations among the samples were visible, show ing possible genetic variability. At 0.89 (89%) coefficient, cluster,~f is made up of 7 samples which are genetically similar. At 0.834 (83.1 %) coefficient, distinct sample BTl2 forms a cluster (~p) with cluster ~f which shows they are related at this coefficient. Cluster ~W (84% coefficient) comprising of 7 samples forms another cluster with a distinct sample BT06 at about 0.79 coefficients. At 78.6% coefficient (cluster ~e). All the samples are genetically similar except sample BT17. This distinct sample can increase genetic variability by a cross between it and other strains of tilapia. Therefore, care should be taken by fish farmers who buy or use the fish samples from this landing site for culture. Proper molecular characterization of this fish species before culture becomes necessary to avoid genetic problems.
| Main Authors: | , |
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| Format: | conference_item biblioteca |
| Language: | English |
| Published: |
FISON
2013
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| Subjects: | Biology, Fisheries, Nigeria, Genetic, Tilapia, RAPD, Badore, freshwater environment, automation, |
| Online Access: | http://hdl.handle.net/1834/39039 |
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