Comparison of diploid and triploid Heterobranchus bidorsalis using Polyacryl Amide Gel Electrophoresis (N-PAGE)
A comparative analysis on biochemistry and Polyacryl Amide Gel Electrophresis was carried out to determine the genetic diversity of diploid and triploid Heterobranchus bidorsalis. Sixteen samples of diploid and triploid farm-raised (mean weight; 512.6g and mean length; 41.6cm) were collected and the electrophoresis analysis was conducted using 5.5% Polyacryl Amide Gel and serum protein obtained from the blood of the live samples. 0.06% Coomassie blue was used for staining the gel while a mixture of ratio 1:2 of glacial acetic acid, meethanol and distilled water was used for de-staining the gel. The diploid and triploid possessed an equal total number of 23 electrophoretic protein bands. The molecular phylogenetics of both samples revealed low genetic variability. Results of this study will serve as a baseline analysis on the current genetic diversity of H. bidorsalis in Nigeria.
| Main Authors: | , , |
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| Format: | conference_item biblioteca |
| Language: | English |
| Published: |
FISON
2009
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| Subjects: | Fisheries, Heterobranchus bidorsalis, Nigeria, Kontagora L., freshwater environment, Genetics, Diploids, Experimental culture, Nutritional requirements, Fish, |
| Online Access: | http://hdl.handle.net/1834/38133 |
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