The hematological improvement of rainbow trout (Oncorhynchus mykiss) during dietary supplementation with vitamin C after exposure to zinc nano-particles
The aim of this study was to examine the adverse effects of zinc nanoparticles on hematological indices of trout and investigate the improvement of these indices after vitamin C treatments. This study assesses the protective role of vitamin C in fish exposed to ZnO NPs. Two concentrations of ZnO-NPs (40 and 80 mg L^-1) and two doses of vitamin C (400 and 800 mg per kg of feed) were used to treat 162 specimens of Oncorhynchus mykiss. No mortality was observed during the test. After 5 and 10 days of exposure, hematological data were analyzed according to routine clinical methods. Statistical analysis showed significant changes in WBCs and RBC on day 10 (p<0.05). Values for HT and MCH were significantly higher in treatment 2 (normal diet+40 mg L^-1 ZnO-NPs) and 9 (800 mg/kg Vit + 80 mg L^-1 ZnO-NPs), and lower in treatment 3 (normal diet+80 mg L^-1 ZnO-NPs) in comparison with the control group (normal diet+0 mg L^-1 ZnO-NPs) (P<0.05). No significant differences of MCV and MCHC were observed (p>0.05), while significant increase in neutrophils and monocytes, and decrease in lymphocyte cells were recorded (p<0.05). ZnO-NPs stimulated the immune system of O. mykiss, but this effect did not have any lethality on this species at 40 and 80 mg L^-1. Vitamin C in different concentrations could help to prevent rainbow trout from the toxic effects of this nano metal.
| Main Authors: | , , , |
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| Format: | article biblioteca |
| Language: | English |
| Published: |
2017
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| Subjects: | Aquaculture, Biology, Fisheries, Pollution, Fish, Hematology, Nano particle, Toxicity, Vitamin C, aquaculture, Iran, |
| Online Access: | http://hdl.handle.net/1834/37765 |
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