Short communication: DNA extraction using liquid nitrogen in Staphylococcus aureus
Nowadays, polymerase chain reaction (PCR) is among the fastest diagnostic way of diseases, in which desoxy nucleic acids (DNA) is an inseparable part for this purpose. There are different approaches to extract the DNA, which are all supported by using the expensive chemical enzymes, however due to lack of access to most of the enzymes and high cost, it is also possible to use substitution cost effective, faster methods, with much more extraction as yield at the end of the procedure. Staphylococcus aureus was considered as the objective species for testing a substitutional method in PCR. Generally, the enzymes used to lyse the cell wall of the Staphylococcus aureus in molecular diagnosis are: motilyzine, lysostafine, lysozyme and etc. In the present study, various approaches such as sonication, freeze-thawing and the liquid nitrogen were used to get cell lysate. The best result was obtained by using liquid nitrogen with -196ºC. S. aureus (RTCC 2411) samples were stored at −20°C and kept until bacteriological test.
| Main Authors: | , , , , , , , |
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| Format: | article biblioteca |
| Language: | English |
| Published: |
2012
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| Subjects: | Biology, Staphylococcus aureus, DNA extraction, Liquid nitrogen, Iran, |
| Online Access: | http://hdl.handle.net/1834/37337 |
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