Investigation of polymorphism by PCR-RFLP method in Cobia fish Rachycentron canadum in the Persian Gulf and Oman Sea
Cobia is a native fish species in Iranian waters in the Persian Gulf and Sea of Omanand has a good internal and foreign market. This fish is a fast growing species and for thisreason Iranian Fisheries is considering to go for it culture practices. To go for any utilizationsuch as fishing from wild stocks or culture activities, needs a better understanding of itspeculiarities and genetic characteristics of its natural resources. Therefore, this project wasdiscribed and conducted.In this investigation, cuts 2 or 3 cm of fin tissue of specimen of Cobia obtainedfrom Sistan and Bluchestan, Hormozgan, Bushehr and Khuzestan water provinces, werecollected. DNA was extracted by Phenol-chlorophorm method and produced PCR product inlength of 1060 and 1450 base pair of two mitochondrial genes COI and NADH2.Using 13 cutting enzymes (4 enzymes were subscriber for both of genes), 205 basepair (from 2510 base pair, equal with %3.8 from gene regains) were directly investigated. Butbinding patterns of enzymatic digestion of PCR products of both COI and ND genes fromelectrophoresis were monomorph in all samples and no polymorphism was observed. Thismay be attributed to the unsuitable choice of COI and ND2 genes for showing of intra specificdivergence. But in general non-existence of genetic diversity or noticeable decrease of thatamong individuals has been reported in regions were fish migration exist and they can freelymove between two regions. Therefore, non-observation of polymorphism in the study areamight be the case and indicates represents the area.On the other hand, some scientists believe that the distributions of populations indifferent regions are greatly affected by environmental and physical and ecological factors.Althoug Cobia is a migratory fish, but with regard to the fact that the environmentalconditions are different (specially temperature and salinity) between east and west of PersianGulf and Oman sea, there is a possibility that different genetic groups of this species exist inthe regions. Of course It is clear that using more samples and enzymes from other geneticallyregions could produce better results.Since none of the two investigated genes didn’t show genetic divergence orpolymorphism amongst the individuals of one region or between different regions, therefore,statistic analysis for estimating of haplotype diversity or nucleotide diversity and drawing ofrelationship tree among individuals using available softwares was not possible.
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| Format: | thesis biblioteca |
| Language: | Persian |
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Islamic Azad University, Science and Research Branch, Tehran, Fisheries
2009
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| Subjects: | Biology, Fisheries, |
| Online Access: | http://hdl.handle.net/1834/34437 |
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