Experimental pathogenesis of Aeromonas hydrophila bacteria in shing Heteropneustes fossilis(Bloch)
Pathogenicity of Aeromonas hydrophila bacteria was tested on the stinging catfishHeteropneustes fossilis. Before artificial infection the morphological, biochemical andphysiological characters of Aeromonas hydrophila were studied. The infections were doneby two different methods, viz., intramuscular (IM) and intraperitoneal (IP) injection. Ininfection experiment, each group of 10 fish were injected either intramuscularly orintra peritoneally with one dose higher than the LD50 dose (9.6 x 107 CFU/fish). All thefish tested died within 1 to 9 days. Both in cases of intramuscular and intraperitonealinjection, external pathology were found. Haemorrhagic lesions were evident at the siteof injection. The posterior end of the body surface was found to develop greyish-whitelesion that was extended up to caudal fin. Hyperemic anal region and the fin bases werealso observed. Total bacterial loads in liver, kidney and intestine were determined.Aeromonas hydrophila could be isolated from liver, kidney and intestine of theexperimentally infected fish. In case of intramuscular injection the highest and thelowest bacterial load was found to be 2.4 x 107 CFU/g of liver and 2.1 x 102 CFU/g ofkidney and in case of intraperitoneal injection they were found to be 3.6 x 106 CFU/g ofkidney and 1.2 x 104 CFU/g of kidney respectively. It was concluded that A. hydrophilacould cause serious disease condition to Heteropneustes fossilis and its pathogenesis in thefish was also very efficient.
| Main Authors: | , , , |
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| Format: | article biblioteca |
| Language: | English |
| Published: |
2008
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| Subjects: | Biology, Fisheries, Experimental pathogenesis, Heteropneustes fossilis, Aeromonas hydrophila, |
| Online Access: | http://hdl.handle.net/1834/33203 |
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