Binding of radio-iodinated gonadotropin to the ovary of exotic carp (Cyprinus carpio Lin.)
Heterologous murrel gonadotropic hormone (m-GtH) binds to common carp oocyte plasma membrane and enhances steroid secretion. With increasing concentration of radio-labelled hormone the receptor binding is also found to increase linearly up to a certain concentration and then decrease. The [¹²⁵I] murrel GtH binding characteristics to a preparation of common carp ovarian plasma membrane shows saturability with high affinity. Scat chard plot analysis gave dissociation constant (Kd) of 0.81 X 10(super -9) M andmaximum binding capacity (MBC) of 22.05 f mole/mg protein.
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| Main Authors: | , |
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| Format: | article biblioteca |
| Language: | English |
| Published: |
2000
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| Subjects: | Biology, radioisotopes, induced breeding, ovaries, freshwater fish, radio-iodinated gonadotropin, sex hormones, Cyprinus carpio, |
| Online Access: | http://hdl.handle.net/1834/32267 |
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