Genetic identification of four Malaysian mackerel species off Coast of Peninsular Malaysia based on molecular marker
Random Amplified Polymorphic DNA (RAPD) markers and cytochrome b (Cyt-b) gene sequences were utilizedto fingerprint and construct phylogenetic relationships among four species of mackerel commonly found in the Straits ofMalacca namely Rastrelliger kanagurta, R. brachysoma, Decapterus maruadsi and D. russelli. The UPGMA dendogramand genetic distance clearly showed that the individuals clustered into their own genus and species except for theDecapterus. These results were also supported by partial mtDNA cytochrome b gene sequences (279 bp) which foundmonotypic sequence for all Decapterus studied. Cytochrome b sequence phylogeny generated through Neighbor Joining(NJ) method was congruent with RAPD data. Results showed clear discrimination between both genera with averagenucleotide divergence about 25.43%. This marker also demonstrated R. brachysoma and R. kanagurta as distinct species separated with average nucleotide divergence about 2.76%. However, based on BLAST analysis, this study indicated that the fish initially identified as D. maruadsi was actually D. russelli. The results highlighted the importance of genetic analysis for taxonomic validation, in addition to morphological traits.
| Main Authors: | , , , , , |
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| Format: | conference_item biblioteca |
| Language: | English |
| Published: |
2009
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| Subjects: | Aquaculture, Biology, Fisheries, |
| Online Access: | http://hdl.handle.net/1834/24458 |
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