Anwendung von Fischzellkulturen in der Meeresforschung
A large number of chemical pollutants can be foundin the marine environment. So it is necessary to obtaininformations about the toxic effects of this contaminantmixtures in general and especially on singlecell level. We used an organic extract of a marine sedimentfrom the North Sea to investigate its cyto- andgenotoxicity with an in vitro system, the comet assayor single cell gel electrophoresis (SCGE). The cometassay can be applied for estimating genotoxic effectsof chemicals on single cell level. First results confirmthe sensitivity of this assay and its applicability in assessing genotoxic load in environmental samples. Apermant cell line, the EPC (Ephithelioma papulosumcyprini) was used for the experiments. It was possibleto demonstrate the suitability of this in vitro test systemfor assessing genotoxic and cytotoxic effects ofmarine sediment extracts on EPC cells.
| Main Authors: | , , |
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| Format: | article biblioteca |
| Language: | German |
| Published: |
2000
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| Subjects: | Ecology, Environment, Chemistry, toxicity, marine fish cells, gentoxicity, |
| Online Access: | http://hdl.handle.net/1834/20996 |
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