Fish gene bank of the Persian Gulf and Oman Sea
Genetic studies and gene banks preparation can identify guidelines for improving diversity and population structure and estimation, poaching and also the amount of cross breeding and provide genetic classification. In this study, sampling was performed from the important studied species habitats areas for the species such as: Dasyatis bennetti ، Netuma thalassina, Netuma bilineata Carcharhinus leucas ،Choerodon robustus ، Pseudorhombus pentophthalmus ،Pseudorhombus arsius Thunnus tonggol ،Euthynnus affinis and Trichiurus lepturus. Total DNA extraction was performed using phenol - chloroform method which is the most common method for DNA extraction in order to achieve high quality of DNA was performed in the preparation of gene bank in this study. After relevant studies on this gene primers were designed and in use. After editing the sequences, nucleotide BLAST (Basic Local Alignment Search Tool) was performed using NCBI blast main page.The sequences obtained from each sample were aligned and corrected from any ambiguities and assembled using Bio edit program .Trees were generated using maximum parsimony (MP), a character-based algorithm and neighbor joining (NJ) a distance-based algorithm for phenetic analysis. The distance matrix option of MEGA4 was used to calculate genetic distance according to the Kimura 2-parameter model of sequence evolution. Based on the results obtained, the optical density of 260 to 280 nm in the samples was recorded between 1/8 - 2, indicating good quality DNA samples. Optimized PCR reaction to 16SrRNA gene amplification using the gradient between 48 - 60° C showed that the most suitable criteria for binding primers, 54 to 58 Celsius degrees respectively.The project objectives including the identification of the genetic structure of the species, and draw the phylogenetic trees using two genes 16SrRNA, making identification and registration of specified computer storage and regulate the structure and management of mentioned species by focus on genetic resources 10 species of majour commercial and noncommercial fishes in the Persia Gulf and Oman Sea through the creation of an integrated network of aquatic genetic resources in the region to try to identify genetic resources and aquatic gene bank.This type of analysis could be considered as an important tool to be used in broodstock selection in breeding programs. Also identify the species of fish named as Pseudorhombus pentophthalmus with a new haplotype and genetic differences 7% in compare to the samples recorded in the World gene Bank can considered as a major achievements of this research.
Main Authors: | , , , , , , , , , , , , , , , , , , , , , , |
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Format: | Report biblioteca |
Language: | Persian |
Published: |
Iranian Fisheries Science Research Institute
2016
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Subjects: | Gene Bank, mtDNA, Fish, Marine, 16S rRNA, Dasyatis bennetti, Netuma thalassina, Netuma bilineata, Carcharhinus leucas, Choerodon robustus, Pseudorhombus pentophthalmus, Pseudorhombus arsius, Thunnus tonggol, Euthynnus affinis, Trichiurus lepturus, Species, |
Online Access: | http://hdl.handle.net/1834/13925 |
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dig-aquadocs-1834-139252021-05-19T06:48:46Z Fish gene bank of the Persian Gulf and Oman Sea Ghoroghi, Ahmad Pourkazemi, M. Rezvani, S. Salehi, Gh. Laloei, F. Hossieni, M.R. Aeinjamshid, Kh. Nazemi, M. Abdolhai, H. Azini, M.R. Ehteshami, F. Aminzadeh, S. Peshevarzad, F. Malolahi, A. Jorfi, E. Safari, R. Safavi, E. Hajrezaei, S. Maghsodlo, E. Aanjari, M. Valinasab, T. Moradi, Y. Hosseinzadeh, H. Gene Bank mtDNA Fish Marine 16S rRNA Dasyatis bennetti Netuma thalassina Netuma bilineata Carcharhinus leucas Choerodon robustus Pseudorhombus pentophthalmus Pseudorhombus arsius Thunnus tonggol Euthynnus affinis Trichiurus lepturus Species Genetic studies and gene banks preparation can identify guidelines for improving diversity and population structure and estimation, poaching and also the amount of cross breeding and provide genetic classification. In this study, sampling was performed from the important studied species habitats areas for the species such as: Dasyatis bennetti ، Netuma thalassina, Netuma bilineata Carcharhinus leucas ،Choerodon robustus ، Pseudorhombus pentophthalmus ،Pseudorhombus arsius Thunnus tonggol ،Euthynnus affinis and Trichiurus lepturus. Total DNA extraction was performed using phenol - chloroform method which is the most common method for DNA extraction in order to achieve high quality of DNA was performed in the preparation of gene bank in this study. After relevant studies on this gene primers were designed and in use. After editing the sequences, nucleotide BLAST (Basic Local Alignment Search Tool) was performed using NCBI blast main page.The sequences obtained from each sample were aligned and corrected from any ambiguities and assembled using Bio edit program .Trees were generated using maximum parsimony (MP), a character-based algorithm and neighbor joining (NJ) a distance-based algorithm for phenetic analysis. The distance matrix option of MEGA4 was used to calculate genetic distance according to the Kimura 2-parameter model of sequence evolution. Based on the results obtained, the optical density of 260 to 280 nm in the samples was recorded between 1/8 - 2, indicating good quality DNA samples. Optimized PCR reaction to 16SrRNA gene amplification using the gradient between 48 - 60° C showed that the most suitable criteria for binding primers, 54 to 58 Celsius degrees respectively.The project objectives including the identification of the genetic structure of the species, and draw the phylogenetic trees using two genes 16SrRNA, making identification and registration of specified computer storage and regulate the structure and management of mentioned species by focus on genetic resources 10 species of majour commercial and noncommercial fishes in the Persia Gulf and Oman Sea through the creation of an integrated network of aquatic genetic resources in the region to try to identify genetic resources and aquatic gene bank.This type of analysis could be considered as an important tool to be used in broodstock selection in breeding programs. Also identify the species of fish named as Pseudorhombus pentophthalmus with a new haplotype and genetic differences 7% in compare to the samples recorded in the World gene Bank can considered as a major achievements of this research. Iranian Fisheries Science Research Institute Published 2018-08-10T09:50:08Z 2018-08-10T09:50:08Z 2016 Report Refereed http://hdl.handle.net/1834/13925 fa 50849; 66pp. Iran Persian Gulf Iranian Fisheries Science Research Institute Tehran, Iran |
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Gene Bank mtDNA Fish Marine 16S rRNA Dasyatis bennetti Netuma thalassina Netuma bilineata Carcharhinus leucas Choerodon robustus Pseudorhombus pentophthalmus Pseudorhombus arsius Thunnus tonggol Euthynnus affinis Trichiurus lepturus Species Gene Bank mtDNA Fish Marine 16S rRNA Dasyatis bennetti Netuma thalassina Netuma bilineata Carcharhinus leucas Choerodon robustus Pseudorhombus pentophthalmus Pseudorhombus arsius Thunnus tonggol Euthynnus affinis Trichiurus lepturus Species |
spellingShingle |
Gene Bank mtDNA Fish Marine 16S rRNA Dasyatis bennetti Netuma thalassina Netuma bilineata Carcharhinus leucas Choerodon robustus Pseudorhombus pentophthalmus Pseudorhombus arsius Thunnus tonggol Euthynnus affinis Trichiurus lepturus Species Gene Bank mtDNA Fish Marine 16S rRNA Dasyatis bennetti Netuma thalassina Netuma bilineata Carcharhinus leucas Choerodon robustus Pseudorhombus pentophthalmus Pseudorhombus arsius Thunnus tonggol Euthynnus affinis Trichiurus lepturus Species Ghoroghi, Ahmad Pourkazemi, M. Rezvani, S. Salehi, Gh. Laloei, F. Hossieni, M.R. Aeinjamshid, Kh. Nazemi, M. Abdolhai, H. Azini, M.R. Ehteshami, F. Aminzadeh, S. Peshevarzad, F. Malolahi, A. Jorfi, E. Safari, R. Safavi, E. Hajrezaei, S. Maghsodlo, E. Aanjari, M. Valinasab, T. Moradi, Y. Hosseinzadeh, H. Fish gene bank of the Persian Gulf and Oman Sea |
description |
Genetic studies and gene banks preparation can identify guidelines for improving diversity and population
structure and estimation, poaching and also the amount of cross breeding and provide genetic classification. In
this study, sampling was performed from the important studied species habitats areas for the species such as:
Dasyatis bennetti ، Netuma thalassina, Netuma bilineata Carcharhinus leucas ،Choerodon robustus ،
Pseudorhombus pentophthalmus ،Pseudorhombus arsius Thunnus tonggol ،Euthynnus affinis and Trichiurus
lepturus.
Total DNA extraction was performed using phenol - chloroform method which is the most common method for
DNA extraction in order to achieve high quality of DNA was performed in the preparation of gene bank in this
study.
After relevant studies on this gene primers were designed and in use. After editing the sequences, nucleotide
BLAST (Basic Local Alignment Search Tool) was performed using NCBI blast main page.The sequences
obtained from each sample were aligned and corrected from any ambiguities and assembled using Bio edit
program .Trees were generated using maximum parsimony (MP), a character-based algorithm and neighbor
joining (NJ) a distance-based algorithm for phenetic analysis. The distance matrix option of MEGA4 was used to
calculate genetic distance according to the Kimura 2-parameter model of sequence evolution.
Based on the results obtained, the optical density of 260 to 280 nm in the samples was recorded between 1/8 - 2,
indicating good quality DNA samples.
Optimized PCR reaction to 16SrRNA gene amplification using the gradient between 48 - 60° C showed that the
most suitable criteria for binding primers, 54 to 58 Celsius degrees respectively.The project objectives including
the identification of the genetic structure of the species, and draw the phylogenetic trees using two genes
16SrRNA, making identification and registration of specified computer storage and regulate the structure and
management of mentioned species by focus on genetic resources 10 species of majour commercial and noncommercial
fishes in the Persia Gulf and Oman Sea through the creation of an integrated network of aquatic
genetic resources in the region to try to identify genetic resources and aquatic gene bank.This type of analysis
could be considered as an important tool to be used in broodstock selection in breeding programs. Also identify
the species of fish named as Pseudorhombus pentophthalmus with a new haplotype and genetic differences 7% in
compare to the samples recorded in the World gene Bank can considered as a major achievements of this
research. |
format |
Report |
topic_facet |
Gene Bank mtDNA Fish Marine 16S rRNA Dasyatis bennetti Netuma thalassina Netuma bilineata Carcharhinus leucas Choerodon robustus Pseudorhombus pentophthalmus Pseudorhombus arsius Thunnus tonggol Euthynnus affinis Trichiurus lepturus Species |
author |
Ghoroghi, Ahmad Pourkazemi, M. Rezvani, S. Salehi, Gh. Laloei, F. Hossieni, M.R. Aeinjamshid, Kh. Nazemi, M. Abdolhai, H. Azini, M.R. Ehteshami, F. Aminzadeh, S. Peshevarzad, F. Malolahi, A. Jorfi, E. Safari, R. Safavi, E. Hajrezaei, S. Maghsodlo, E. Aanjari, M. Valinasab, T. Moradi, Y. Hosseinzadeh, H. |
author_facet |
Ghoroghi, Ahmad Pourkazemi, M. Rezvani, S. Salehi, Gh. Laloei, F. Hossieni, M.R. Aeinjamshid, Kh. Nazemi, M. Abdolhai, H. Azini, M.R. Ehteshami, F. Aminzadeh, S. Peshevarzad, F. Malolahi, A. Jorfi, E. Safari, R. Safavi, E. Hajrezaei, S. Maghsodlo, E. Aanjari, M. Valinasab, T. Moradi, Y. Hosseinzadeh, H. |
author_sort |
Ghoroghi, Ahmad |
title |
Fish gene bank of the Persian Gulf and Oman Sea |
title_short |
Fish gene bank of the Persian Gulf and Oman Sea |
title_full |
Fish gene bank of the Persian Gulf and Oman Sea |
title_fullStr |
Fish gene bank of the Persian Gulf and Oman Sea |
title_full_unstemmed |
Fish gene bank of the Persian Gulf and Oman Sea |
title_sort |
fish gene bank of the persian gulf and oman sea |
publisher |
Iranian Fisheries Science Research Institute |
publishDate |
2016 |
url |
http://hdl.handle.net/1834/13925 |
work_keys_str_mv |
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