Morphological changes of mitochondria-rich cells in gill of Sparidentex hasta during adaptation to different environmental salinities by scanning electron microscopy
To determine the cellular and adaptations changes in mitochondria-rich cells under different salinities, Sparidentex hasta as a urihaline has been studied.180 fish weighing 150 ± 0.5 gr and 23±1 cm length, were directly exposed to (5 , 20 , 40 and 60 ppt) salinities for a week. Sampling was performed on days 1, 2 and 7 and three fish from each tank (12 fish from each treatment) were sampled each time. Samples were fixed in glutaraldehyde 2/5 % solution and washed with 0.1M (pH=7.4) buffer phosphate. Samples were dehydrated in increasing ethanol series (from 50 % to pure ethanol ) followed by 100 % acetone. Tissue blocks were frozen by liquid nitrogen and then immediately followed by scanning electron microscope LEO (Model 1455VP) with 15Kv voltage. Scanning electron microscope revealed three types of mitochondria-rich cells with derived apertures ,shallow and deep apical openings. The number and size of mitochondria-rich cells in the apical openings in 60ppt salinity increased on the first day compared to rest of the treatments. During the experiment, the density and size of the openings in the mitochondria-rich cells in the lower salinity increased but in higher salinity of sea water size and density of openings decreased during experiment.
| Main Authors: | , , |
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| Format: | Journal Contribution biblioteca |
| Language: | Persian |
| Published: |
2016
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| Subjects: | Morphological, Mitochondria-rich cells, Scanning electron microscopy, Sparidentex hasta, Salinitiy, Gill, |
| Online Access: | http://hdl.handle.net/1834/13640 |
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