Cryopreservation of sperm in Acipenser nudiventris, Huso huso, Acipenser stellatus and Acipenser persicus in the breeding season
Considering decrease in total catch of sturgeon & threat of extinction in their stocks, special measures might be adopted. Sperm cryopreservation is one of the suitable methods to prepare bank of frozen gamete for future use in artificial breeding in order to prohibit extinction of sturgeon stocks. This study carried out on 27 male sturgeon during 2001-2004. The investigated sturgeon include 12 male Persian sturgeon (Acipenser persicus), 7 male Acipenser stellatus, 5 male Acipenser nudiventris and 3 male Huso huso. Sperm Collected from spawners in Shahid Beheshti & Shahid Marjani sturgeon rearing & propagation Complex in Rasht & Gorgan. The Sperm which was collected from Shahid Marjani propagation complex placed in the sealed Vessels & transferred by coleman in near zero temperature to cryopreservation laboratory of international sturgeon research institute for further investigation. In this study, the sperm was diluted in ratio 1:1 in two culture media containing dimethyl sulfoxide & glycerol (BC) and the samples stored in 1 ml insulin syringe & 0.5 ml Piot. The diluted sperm froze in a special temperature by automated freezer model 5300 (France IMV). Three phases applied to freeze the samples which are as follow: 1- Begin to freeze from +50c to -100c (30c /min) 2- From 10 0c to 70 0c (20 0c /min) 3- From 70 0c to 130 0c (25 0c /min) After freezing, the samples placed in liquid nitrogen containers with 196 0c temperature. For thawing, the sperm samples took out of liquid nitrogen & placed in water 40 0c. Then motility percent & the sperm quality investigated under 400x microscope. According to the results the mean motility percentage of fresh sperm in Persian sturgeon, Acipenser Stellatus, Acipenser nudiventris and Huso huso was 84, 73.75, 67.5 and 76.66, respectively. The mean percentage of motility in frozen sperm which placed in media containing dimethyl sulfoxide was 32, 37.5, 40 and 20%, respectively. Also, the frozen samples that preserved in BC media (Biociphus) showed 5.2, 75.25, 4.11 and 2.66% motility, respectively. In blank group, the mean fertilization percent of eggs was 90, 72, 71.25 and 90%, respectively. In the treatment group applying frozen sperm in culture media containing dimetlylsulfoxide, the mean fertilization rate was 30, 6.5, 25.39 and 4.75%. Furthermore, no fertilization (0.0%) observed using frozen sperm stored in Biociphus culture media containing glycerol. There was no significant difference in fertilization percent comparing the two storage places (syringe & Piot). According to investigations the culture media containing dimethyl sulfoxide, is a suitable diluter for sturgeon sperm. So, the Cryopreservation technique can be used to preserve the sturgeon sperm for future fertilization & through this way we can prevent extinction of sturgeon stocks.
| Main Authors: | , , |
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| Format: | Report biblioteca |
| Language: | Persian |
| Published: |
Iranian Fisheries Science Research Institute
2007
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| Subjects: | Cryopreservation, Sperm, Acipenser nudiventris, Huso huso, Acipenser stellatus, Acipenser persicus, Breeding, Sturgeon, Temperature, Motility, Fertilization, Spermatozoa, Freezing, |
| Online Access: | http://hdl.handle.net/1834/13044 |
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