Investigation of genetic structure in populations of Mnemiopsis leidyi from north and south of Caspian Sea using RAPE Method
The molecular structure in populations of Mnemiopsis leidyi were examined for 200 samples from the south (Guilan, Mazandaran and Golestan provinces) and north of the Caspian Sea. DNA was extracted from M leiydi tissue by phenol-chiorophorm method and its concentration was found at 50 to 10Ong. We used the PCR method using 19 random primers (10bp). The PCR products of samples were accompanied with standard marker (50bp DNA ). To measure fragment size, samples were run on a 1% agarose gel. Ten of the ninteen primers showed polymorphism. Statistical analysis of data was performed using POPGENE software. The avarage genetic variation was 0.189 in total samples and the maximum variation was found in samples from north of the Caspian Sea. Also, The maximume genetic distance was between north of the Sea and Golestan coasts in the south (0.089). The minimum genetic distance was between Mazandaran and Guilan coasts (0.001). The UOGMA dendogram showed two clusters. The samples of Mazandaran, Guilan and Golestan coasts were placed in one cluster and samples of the north area in another. The genetic diversity was significantly different between samples of the north area and Golestan coasts (P<0.05). We found a significant genetic divergence between some samples and therefore suggested at least two genetic groups of Mnemiopsis leidyi in the Caspian Sea.
| Main Authors: | , , |
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| Format: | Journal Contribution biblioteca |
| Language: | Persian |
| Published: |
2010
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| Subjects: | Mnemiopsis leidyi, RAPD, Diversity, Genetic, Populations, DNA, |
| Online Access: | http://hdl.handle.net/1834/11750 |
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