The Azov anchovy proteins enzymatic hydrolysis modes development

The research was aimed to assess the Azov anchovy proteins enzymatic hydrolysis effectiveness using industrial photolytic enzyme agent protosubtilin G3x. Raw fish chemical composition was clarified, protein enzymatic hydrolysis products accumulation dynamic at various process conditions was studied, enzymatic hydrolysis optimum parameters for different process indicators were determined. Raw fish chemical composition studies detected high fat amount (near 14 %) with proteins content. As a result of a three-factor orthogonal central composite experimental design application while determining Azov anchovy proteins enzymatic hydrolysis modes, second order adequate models were received. Enzymatic hydrolysis process parameters optimization using simplex method with regression equations maximization as objective function showed that the low molecular protein fragments maximum accumulation is observed at hydrolysis duration 403 minutes, hydronic module – 7 % and enzyme drag dose 0.22 %. To maximize the accumulation of heat-resistant protein fragments in Azov anchovy hydrolysates, enzymolysis optimal modes should be: process duration – 403 min, enzyme agent dosage – 0.59 % and high hydronic module – 56 %. The maximum protein hydrolysis degree can be obtained by enzymatic hydrolysis duration 403 minutes, enzyme agent dose 0.50 % and hydronic module equal to 4.0 %. The revealed differences of optimum process modes for accepted indicators are obviously connected with the complex collection of enzymatic processes in multi-component enzyme-substrate system for which diffusion and multi-stage competitive enzyme inhibition by various reaction products is possible.

Saved in:
Bibliographic Details
Main Authors: Vinnov, A.S., Chernyavskaya, S.L., Krivonos, O.N.
Other Authors: Panov, Boris
Format: Journal Contribution biblioteca
Language:English
Published: 2017
Subjects:Enzymatic hydrolysis, Azov anchovy, Regression equations, Enzyme agents, Proteins, Fish processing, Protein hydrolysate, Chemical composition, Drag dose,
Online Access:http://hdl.handle.net/1834/11030
Tags: Add Tag
No Tags, Be the first to tag this record!