Molecular diagnosis optimization of virus, bacteria and fungi in sugarcane.
Abstract: Aiming at optimizing the diagnosis of major sugarcane diseases by PCR, primers were developed (scald, orange rust) and amplified fragments according to the literature were used as positive control, such as those caused by: 1 - Bacteria, (ratoon stunting and leaf scald) 2 - Viruses, (yellow leaf, mosaic, mosaic streak and fijivirus) 3-Fungus, (smut, orange rust and curvularia). For diseases with long latency period (leaf scald, ratoon stunting, yellow leaf, mosaic, fijivirus, smut and orange rust), primers were designed for real time PCR. For testing, infected leaves, fungal colonies, amplified DNA fragment or 40 seedlings were used. Real time PCR analyses have enabled the detection of sugarcane samples with highest dilution of DNA. The pair of primers designed for orange rust was more specific. The pair of primers designed for scald, apparently was not able to distinguish only bacteria of the genus Erwinia, whereas the primers described in the literature were observed amplify others genera, such as Xanthomonas, Pseudomonas, Erwinia, Stenotrophomona and Pantoea. The three strains of the fungus curvularia, found in sugarcane, resembled more to the species C.cymbopogonis, C.geniculata, C.sp. Of the 28 primer pairs tested, the best were shown for each of the disease-causing agent.
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Separatas biblioteca |
| Language: | English eng |
| Published: |
2013-08-01
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| Subjects: | Leaf scald, PCR, Cana de açúcar, Doença de planta, Diagnóstico, Bactéria, Vírus, Fungo, Escaldadura, Ferrugem alaranjada, Plant diseases and disorders, Sugarcane, Ratoon stunting disease, Scald diseases, Sugarcane yellow leaf virus, Sugarcane streak virus, Fijivirus, Smut diseases, Rust diseases, Curvularia, Polymerase chain reaction, |
| Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/963146 |
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