Systemic transport of Alfalfa mosaic virus can be mediated by the movement proteins of several viruses assigned to five genera of the 30K family.

We previously showed that the movement protein (MP) gene of Alfalfa mosaic virus (AMV) is functionally exchangeable for the cell-to-cell transport of the corresponding genes of Tobacco mosaic virus (TMV), Brome mosaic virus, Prunus necrotic ringspot virus, Cucumber mosaic virus and Cowpea mosaic virus. We have analysed the capacity of the heterologous MPs to systemically transport the corresponding chimeric AMV genome. All MPs were competent in systemic transport but required the fusion at their C terminus of the coat protein-interacting Cterminal 44 aa (A44) of the AMV MP. Except for the TMV MP, the presence of the hybrid virus in upper leaves correlated with the capacity to move locally. These results suggest that all the MPs assigned to the 30K superfamily should be exchangeable not only for local virus movement but also for systemic transport when the A44 fragment is present.

Bibliographic Details

Main Authors:	FAJARDO, T. V. M., PEIRÓ, A., PALLÁS, V., SÁNCHEZ-NAVARRO, J.
Other Authors:	THOR VINICIUS MARTINS FAJARDO, CNPUV; Ana Peiró, Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia; Vicente Pallás, Instituto de Biologia Molecular y Celular de Plantas, Universidad Politécnica de Valencia; Jesús Sánchez-Navarro, Instituto de Biologia Molecular y Celular de Plantas, Universidad Politecnica de Valencia.
Format:	Artigo de periódico biblioteca
Language:	English eng
Published:	2013-04-29
Subjects:	Expressão genética, Alfafa, Doença de planta, Vírus,
Online Access:	http://www.alice.cnptia.embrapa.br/alice/handle/doc/956777
Tags:	Add Tag No Tags, Be the first to tag this record!