Development and validation of a method for the analysis of Ochratoxin A in roasted coffee by liquid chromatography/electrospray-mass spectrometry in Tandem (LC/ESI-MS/MS).

A method using LC/ESI-MS/MS for the quantitative analysis of Ochratoxin A in roasted coffee was described. Linearity was demonstrated (r = 0.9175). The limits of detection and quantification were 1.0 and 3.0 ng g-1, respectively. Trueness, repeatability and intermediate precision values were 89.0-108.8%; 2.4-13.7%; 12.5-17.8%, respectively. To the best of our knowledge, this is the first report in which Ochratoxin A in roasted coffee is analysed by LC/ESI-MS/MS, contributing to the field of mycotoxin analysis, and it will be used for future production of Certified Reference Material.

Bibliographic Details

Main Authors:	BANDEIRA, R. D. C. C., UEKANE, T. M., CUNHA, C. P. da, GEAQUINTO, L. R. O., CUNHA, V. S., CAIXEIRO, J. M. R., GODOY, R. L. O., CRUZ, M. H. C. de la.
Other Authors:	RAQUEL D. C. C. BANDEIRA, INMETRO; THAIS M. UEKANE, INMETRO; CAROLINA P. DA CUNHA, INMETRO; LUTHS R. O. GEAQUINTO, INMETRO; VALNEI S. CUNHA, INMETRO; JANAINA M. R. CAIXEIRO, INMETRO; RONOEL LUIZ DE OLIVEIRA GODOY, CTAA; MARCUS HENRIQUE C. DE LA CRUZ, INMETRO.
Format:	Separatas biblioteca
Language:	English eng
Published:	2012-08-17
Subjects:	Ocratoxina A, Cromatografia líquida, Espectrometria de massa., Café.,
Online Access:	http://www.alice.cnptia.embrapa.br/alice/handle/doc/931443
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