Development of mutants of Coniothyrium minitans with improved efficiency for control of Sclerotinia sclerotiorum.
Coniothurium minitans (CM) is hyperparasitic to Sclerotinia sderotiorum (55), a palhogen of many economically important crops. In this paper, we describe lhe isolation of improved mutants of CM, using a UV - irradiation regime, with altered chitinase production and tolerance to high concentration of iprodione, which are effect:iveagainst Ss. Three out of the 59 mutants obtained inhibited the mycelial growth of CM. Infectivity of sclerotia by the new mutants was assayed by the plant-tissue-based system using carrot segments. More than 80%of sclerotia were colonized by lhe mutants and the wild-type CM. The mutant strains retained ability to produce significant amounts of chitinase. The mutants differed frorn their wild-type strain in appearance, morphology and sporulation. In conclusion, the results presented here provide evidence that lhe new biotypes of C. minitans are effective in controlling S.sclemtiarum.
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Artigo de periódico biblioteca |
| Language: | English eng |
| Published: |
2011-08-18T11:11:11Z
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| Subjects: | Coniothvrium minitans, Resistance, Antibiotic production., Sclerotinia sclerotiorum, Doença fúngica, Controle biológico, Fungo para controle biológico, Biological control, Antifungal agents, iprodione., |
| Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/898276 |
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