Coffea expansin gene family and expansin expression during fruit maturation.
Expansins (EXP) are plant cell-wall loosing proteins involved in cell enlargement and developmental process such as organogenesis, seeds germination, cell wall dissolution and fruit ripening. Two families of EXP are known, α-expansin (EXPA), involved in the control of cell wall extension, and β-expansins (EXPB), the major allergens of grass pollen that also have cell wall-loosening activity. With the objective to study the role of EXP in coffee fruit maturation we selected EXP homologous sequences on the Brazilian Coffee Genome Project database. Full-length contigs were classified according the EXP family. Northern blots of pulp from fruits at the latest stages of maturation (22-32 weeks after flowering) showed increased transcription of one contig (CaEXP1). This higher transcription corresponds with a climacteric burst, during the expansion of pulp, with a decline in ?cherry? fruits. The transcription of CaEXP1 was also observed in roots, shoots, flower and flower buds, but not in leaves.
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Format: | Anais e Proceedings de eventos biblioteca |
Language: | English eng |
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2011-04-13
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Subjects: | Oganogenesis., Coffea., |
Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/885761 |
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dig-alice-doc-8857612013-09-20T08:52:39Z Coffea expansin gene family and expansin expression during fruit maturation. BUDZINSKI, I. G. F. LEITE, T. F. TAKAHASHI, E. K. PEREIRA, L. F. P. VIEIRA, L. G. E. IAPAR; IAPAR; ÁGUAS CLARAS BIOTECNOLOGIA LTDA.; LUIZ FILIPE PROTASIO PEREIRA, SAPC; IAPAR. Oganogenesis. Coffea. Expansins (EXP) are plant cell-wall loosing proteins involved in cell enlargement and developmental process such as organogenesis, seeds germination, cell wall dissolution and fruit ripening. Two families of EXP are known, α-expansin (EXPA), involved in the control of cell wall extension, and β-expansins (EXPB), the major allergens of grass pollen that also have cell wall-loosening activity. With the objective to study the role of EXP in coffee fruit maturation we selected EXP homologous sequences on the Brazilian Coffee Genome Project database. Full-length contigs were classified according the EXP family. Northern blots of pulp from fruits at the latest stages of maturation (22-32 weeks after flowering) showed increased transcription of one contig (CaEXP1). This higher transcription corresponds with a climacteric burst, during the expansion of pulp, with a decline in ?cherry? fruits. The transcription of CaEXP1 was also observed in roots, shoots, flower and flower buds, but not in leaves. 2011-04-13T11:11:11Z 2011-04-13T11:11:11Z 2011-04-13 2007 2011-04-13T11:11:11Z Anais e Proceedings de eventos In:INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 21., 2006, Montpellier, France. Table of contents... Montpellier, France: Association for Science and Information on Coffee, 2007. 1 CD-ROM. http://www.alice.cnptia.embrapa.br/alice/handle/doc/885761 en eng openAccess |
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Oganogenesis. Coffea. Oganogenesis. Coffea. |
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Oganogenesis. Coffea. Oganogenesis. Coffea. BUDZINSKI, I. G. F. LEITE, T. F. TAKAHASHI, E. K. PEREIRA, L. F. P. VIEIRA, L. G. E. Coffea expansin gene family and expansin expression during fruit maturation. |
description |
Expansins (EXP) are plant cell-wall loosing proteins involved in cell enlargement and developmental process such as organogenesis, seeds germination, cell wall dissolution and fruit ripening. Two families of EXP are known, α-expansin (EXPA), involved in the control of cell wall extension, and β-expansins (EXPB), the major allergens of grass pollen that also have cell wall-loosening activity. With the objective to study the role of EXP in coffee fruit maturation we selected EXP homologous sequences on the Brazilian Coffee Genome Project database. Full-length contigs were classified according the EXP family. Northern blots of pulp from fruits at the latest stages of maturation (22-32 weeks after flowering) showed increased transcription of one contig (CaEXP1). This higher transcription corresponds with a climacteric burst, during the expansion of pulp, with a decline in ?cherry? fruits. The transcription of CaEXP1 was also observed in roots, shoots, flower and flower buds, but not in leaves. |
author2 |
IAPAR; IAPAR; ÁGUAS CLARAS BIOTECNOLOGIA LTDA.; LUIZ FILIPE PROTASIO PEREIRA, SAPC; IAPAR. |
author_facet |
IAPAR; IAPAR; ÁGUAS CLARAS BIOTECNOLOGIA LTDA.; LUIZ FILIPE PROTASIO PEREIRA, SAPC; IAPAR. BUDZINSKI, I. G. F. LEITE, T. F. TAKAHASHI, E. K. PEREIRA, L. F. P. VIEIRA, L. G. E. |
format |
Anais e Proceedings de eventos |
topic_facet |
Oganogenesis. Coffea. |
author |
BUDZINSKI, I. G. F. LEITE, T. F. TAKAHASHI, E. K. PEREIRA, L. F. P. VIEIRA, L. G. E. |
author_sort |
BUDZINSKI, I. G. F. |
title |
Coffea expansin gene family and expansin expression during fruit maturation. |
title_short |
Coffea expansin gene family and expansin expression during fruit maturation. |
title_full |
Coffea expansin gene family and expansin expression during fruit maturation. |
title_fullStr |
Coffea expansin gene family and expansin expression during fruit maturation. |
title_full_unstemmed |
Coffea expansin gene family and expansin expression during fruit maturation. |
title_sort |
coffea expansin gene family and expansin expression during fruit maturation. |
publishDate |
2011-04-13 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/885761 |
work_keys_str_mv |
AT budzinskiigf coffeaexpansingenefamilyandexpansinexpressionduringfruitmaturation AT leitetf coffeaexpansingenefamilyandexpansinexpressionduringfruitmaturation AT takahashiek coffeaexpansingenefamilyandexpansinexpressionduringfruitmaturation AT pereiralfp coffeaexpansingenefamilyandexpansinexpressionduringfruitmaturation AT vieiralge coffeaexpansingenefamilyandexpansinexpressionduringfruitmaturation |
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