Coffea expansin gene family and expansin expression during fruit maturation.

Expansins (EXP) are plant cell-wall loosing proteins involved in cell enlargement and developmental process such as organogenesis, seeds germination, cell wall dissolution and fruit ripening. Two families of EXP are known, α-expansin (EXPA), involved in the control of cell wall extension, and β-expansins (EXPB), the major allergens of grass pollen that also have cell wall-loosening activity. With the objective to study the role of EXP in coffee fruit maturation we selected EXP homologous sequences on the Brazilian Coffee Genome Project database. Full-length contigs were classified according the EXP family. Northern blots of pulp from fruits at the latest stages of maturation (22-32 weeks after flowering) showed increased transcription of one contig (CaEXP1). This higher transcription corresponds with a climacteric burst, during the expansion of pulp, with a decline in ?cherry? fruits. The transcription of CaEXP1 was also observed in roots, shoots, flower and flower buds, but not in leaves.

Bibliographic Details

Main Authors:	BUDZINSKI, I. G. F., LEITE, T. F., TAKAHASHI, E. K., PEREIRA, L. F. P., VIEIRA, L. G. E.
Other Authors:	IAPAR; IAPAR; ÁGUAS CLARAS BIOTECNOLOGIA LTDA.; LUIZ FILIPE PROTASIO PEREIRA, SAPC; IAPAR.
Format:	Anais e Proceedings de eventos biblioteca
Language:	English eng
Published:	2011-04-13
Subjects:	Oganogenesis., Coffea.,
Online Access:	http://www.alice.cnptia.embrapa.br/alice/handle/doc/885761
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