Detection of coffee ringspot virus (CoRSV) in the mite vector by RT-qPCR.

The mite Brevipalpus phoenicis (Geijskes, 1939) (Acari: Tenuipalpidae) is responsible for the transmission of coffee ringspot. There is a great interest in the study of Brevipalpus transmitted viruses (BrTVs), and the interaction between them, their vectors and hosts. Electron microscopy analyses suggest that CoRSV replicates in the mite tissue, what would characterize a persistent propagative virus-vector relationship. This study aimed to establish a transcriptase quantitative polymerase chain reaction (RT-qPCR) method to evaluate and compare the replication capacity of the virus in the vector. Total RNA was extracted from a sample of 100 mites reared in symptomatic plants for CoRSV using two different methods: CTAB and Nucleo Spin RNA XS Kit (Macherey-Nagel) and quantified in a NanoDrop 8000 (Thermo Scientific).

Bibliographic Details

Main Authors:	COSTA, F. M., BASTIANEL, M., FREITAS-ASTUA, J., KUBO, K. S., MACHADO, M. A., GONDIM JUNIOR, M. G. C.
Other Authors:	F. M. Costa, UFRPE; Marinês Bastianel, APTA; JULIANA DE FREITAS ASTUA, CNPMF; Karen Sumire Kubo, IAC; Marcos Antonio Machado, APTA; Manoel Guedes Corrêa Gondim Júnior, APTA.
Format:	Anais e Proceedings de eventos biblioteca
Language:	Ingles English
Published:	2011-03-22
Subjects:	Brevipalpus Phoenicis, Vírus,
Online Access:	http://www.alice.cnptia.embrapa.br/alice/handle/doc/881980
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