Polymorphic information content of SSR markers for Coffea spp.

Polymorphic information content of SSR markers for Coffea spp.

Thirty-three coffee SSR primers from enriched genomic library with (GT)15 and (AGG)10 repeats were analyzed in 24 coffee tree accessions. Twenty-two primers were polymorphic among accessions; the number of alleles ranged from 2 to 13, with the mean number of 5.1 alleles per primer. PIC values ranged from 0.08 to 0.79. The highest mean PIC values were found for C. canephora (0.46), and the lowest values for C. arabica (0.22) and triploids (0.22) accessions. The polymorphic SSR markers used in this study were useful for genetic fingerprinting in the coffee tree, especially in the C. canephora and the leaf rust resistant arabica cultivars.

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Bibliographic Details
Main Authors: MISSIO, R. F., CAIXETA, E. T., ZAMBOLIM, E. M., CRUZ, C. D., SAKIYAMA, N. S.
Other Authors: ROBSON FERNANDO MISSIO, Universidade Federal do Paraná; EVELINE TEIXEIRA CAIXETA, SAPC; EUNIZE MACIEL ZAMBOLIM, Universidade Federal do Paraná; COSME DAMIÃO CRUZ, Universidade Federal de Viçosa; NEY SUSSUMU SAKIYAMA, Universidade Federal de Viçosa.
Format: Artigo de periódico biblioteca
Language:pt_BR
por
Published: 2011-03-11
Subjects:SSR., Arabica coffee, Genomic DNA libraries enriched, Molecular mrkers,
Online Access:http://www.alice.cnptia.embrapa.br/alice/handle/doc/880497
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