Gene flow from transgenic common beans expressing the bar gene.
Gene flow is a common phenomenon even in self-pollinated plant species. With the advent of genetically modified plants this subject has become of the utmost importance due to the need for controlling the spread of transgenes. This study was conducted to determine the occurrence and intensity of outcrossing in transgenic common beans. In order to evaluate the outcross rates, four experiments were conducted in Santo Antonio de Goiás (GO, Brazil) and one in Londrina (PR, Brazil), using transgenic cultivars resistant to the herbicide glufosinate ammonium and their conventional counterparts as recipients of the transgene. Experiments with cv. Olathe pinto and the transgenic line Olathe M1/4 were conducted in a completely randomized design with ten replications for three years in one location, whereas the experiments with cv. Pérola and the transgenic line Pérola M1/4 were conducted at two locations for one year, with the transgenic cultivar surrounded on all sides by the conventional counterpart. The outcross occurred at a negligible rate of 0.00741% in cv. Pérola, while none was observed (0.0%) in cv. Olathe pinto. The frequency of gene flow was cultivar dependent and most of the observed outcross was within 2.5 m from the edge of the pollen source.
| Main Authors: | , , |
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| Other Authors: | |
| Format: | Artigo de periódico biblioteca |
| Language: | English eng |
| Published: |
2010-08-20
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| Subjects: | Fluxo genético, Feijão, Phaseolus vulgaris, Cruzamento, Planta transgênica, Engenharia genética, Beans, Gene flow, Glufosinate, Outcrossing, |
| Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/860572 |
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