Discrimination of Rhizobium tropici and R. leguminosarum strains by PCR-specific amplification of 16S-23S rDNA spacer region fragments and denaturing gradient gel electrophoresis (DGGE).

With the aim of detecting Rhizobium species directly in the environment, specific PCR primers for Rh. tropici and Rh, leguminosarum were designed on the basis of sequence analysis of 16S-23S rDNA spacer regions of several Rh, tropici, Rh. leguminosarum and Agrobacterium rhizogenes strains. Primer specificity, was checked by comparison with available rDNA spacer sequences in databases, and by PCR using DNA from target and reference strains. Sequence polymorphisms of rDNA spacer fragments electrophoresis (DGGE). The specific PCR primers, designed in this study could be applied to evaluate the diversity of Rh, tropici and Rh, leguminosarum by analysing the polymorphisms of 16S-23S spacer rDNA amplified from either whole-cell or soil-extracted DNA.

Bibliographic Details

Main Authors:	OLIVEIRA, V. M. de, COUTINHO, H. L. da C., SOBRAL, B. W. S., GUIMARAES, C. T., ELSAS, J. D. van, MANFIO, G. P.
Other Authors:	V. M. DE OLIVEIRA, FUNDAÇÃO ANDRÉ TOSELLO; HEITOR LUIZ DA COSTA COUTINHO, CNPS; B. W. S. SOBRAL, NATIONAL CENTER FOR GENOME RESOURCES; C. T. GUIMARÃES, NATIONAL CENTER FOR GENOME RESOURCES; J. D. VAN ELSAS, RESEARCH INSTITUTE FOR PLANT PROTECTION; GILSON PAULO MANFIO, FUNDAÇÃO ANDRÉ TOSELLO.
Format:	Artigo de periódico biblioteca
Language:	Ingles English
Published:	2002-11-22
Subjects:	Rhizobium,
Online Access:	http://www.alice.cnptia.embrapa.br/alice/handle/doc/335364 https://doi.org/10.1046/j.1365-2672.1999.00480.x
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